Seamless Cloning with In-Fusion Cloning Kits—No Ligation
Change the way you think about cloning! Eliminate subcloning forever, and never add an unnecessary base to your project. Just one quick reaction allows you to recover your final construct. Use In-Fusion HD Cloning Plus kits to seamlessly and directionally clone one or more fragments into any vector, with high accuracy and high fidelity. This versatile system easily outpaces any traditional cloning method, including ligation and TA cloning:
|Save Time||Be Efficient||Don’t Compromise on Flexibility|
|One 15 min. reaction for single or multiple fragments||Single insert cloning yields 95–100%||Clone any insert into any location within any vector|
|No additional treatment of PCR fragments required||Multiple insert cloning yields 90–100%||Seamless cloning—no extra base pairs left over|
|No subcloning—get your final construct on the 2nd day||Large inserts or vectors are not a problem—clone directly into your expression plasmid||Primer design lets you easily preserve or eliminate restriction sites at cloning junctions|
Do I want to use Cloning Enhancer (CE)?
|Insert PCR produces a single clean band; CE removes background plasmid DNA and PCR residue prior to cloning||Insert PCR produces more than one product; NucleoSpin Gel and PCR Clean-Up kits are suitable for PCR purification and gel extraction|
Do I want the Liquid or EcoDry format?
|Feature||In-Fusion HD EcoDry Kits||In-Fusion HD Liquid Kits|
|Pre-aliquoted, lyophilized components that minimize handling errors|
|Room temperature storage, saving freezer space and eliminating freeze-thaw cycles|
|Ability to customize: reaction volumes and/or plasticware|
All In-Fusion HD Cloning Plus Kits contain CloneAmp HiFi PCR premix, In-Fusion HD enzyme premix, and reagents for control experiments (linearized vector, 2kb insert).