LOCUS pTetOne-Luc 5825 bp DNA circular SYN 21-AUG-2013 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Catalog Nos. 634301, 634302, 634303 SOURCE synthetic DNA construct FEATURES Location/Qualifiers promoter complement(1..365) /label="TRE3Gs promoter" misc_feature 372..525 /label="Transcription blocker" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene combined with a synthetic polyA signal" promoter 545..1055 /label="hPGK promoter" CDS 1074..1820 /label="Tet-On 3G" polyA_signal 1937..2018 /label="SV40 polyA signal" rep_origin complement(2200..2788) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2959..3819) /label="AmpR" promoter complement(3820..3924) /label="AmpR promoter" polyA_signal 3957..4038 /label="SV40 polyA signal" CDS complement(4164..5816) /label="luciferase" /note="firefly luciferase" ORIGIN 1 tttacgaggg taggaagtgg tacggaaagt tggtataaga caaaagtgtt gtggaattga 61 agtttactca aaaaatcagc actcttttat aggcgccctg gtttacataa gcaaagctta 121 tacgttctct atcactgata gggagtaaac tggatatacg ttctctatca ctgataggga 181 gtaaactgta gatacgttct ctatcactga tagggagtaa actggtcata cgttctctat 241 cactgatagg gagtaaactc cttatacgtt ctctatcact gatagggagt aaagtctgca 301 tacgttctct atcactgata gggagtaaac tcttcatacg ttctctatca ctgataggga 361 gtaaactcga gagagaaatg ttctggcacc tgcacttgca ctggggacag cctattttgc 421 tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta tcgattcaca 481 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattctcga ggtgataatt 541 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct 601 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca 661 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc 721 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac 781 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg 841 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga 901 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt 961 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct 1021 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta 1081 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg 1141 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact 1201 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc 1261 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg 1321 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca 1381 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc 1441 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg 1501 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca 1561 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg 1621 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt 1681 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg 1741 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc 1801 ttgacatgct ccccgggtaa atctccagag gatcataatc agccatacca catttgtaga 1861 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 1921 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 1981 catcacaaat ttcacaaata aagcattttt ttcactgccc cgagcttcct cgctcactga 2041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 2341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 2401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 2461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 2521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 2581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 2641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 2701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 2761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 2821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 2881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 2941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 3001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 3061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 3301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 3361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 3421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 3481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 3541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 3601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 3661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 3721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 3781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 3841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 3901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag 3961 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 4021 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 4081 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 4141 gatcctctag acatatgctg cagttacaat ttggactttc cgcccttctt ggcctttatg 4201 aggatctctc tgatttttct tgcgtcgagt tttccggtaa gacctttcgg tacttcgtcc 4261 acaaacacaa ctcctccgcg caactttttg gcggttgtta cttgactggc gacgtaatcc 4321 acgatctctt tttccgtcat cgtctttccg tgctccaaaa caacaacggc ggcgggaagt 4381 tcaccggcgt catcgtcggg aagacctgcc acgcccgcgt cgaagatgtt ggggtgttgt 4441 aacaatatcg actccaattc agcgggggcc acctggtatc ctttgtattt aattaaagac 4501 ttcaagcggt caactatgaa gaagtgttcg tcttcgtccc agtaagctat gtctccagaa 4561 tgtagccatc catccttgtc aatcaaggcg ttggtcgctt ccggattgtt tacataaccg 4621 gacataatca taggtcctct gacacataat tcgcctctct gattaacgcc cagcgttttc 4681 ccggtatcca gatccacaac cttcgcttca aaaaatggaa caactttacc gaccgcgccc 4741 ggtttatcat ccccctcggg tgtaatcaga atagctgatg tagtctcagt gagcccatat 4801 ccttgtcgta tccctggaag atggaagcgt tttgcaaccg cttccccgac ttctttcgaa 4861 agaggtgcgc ccccagaagc aatttcgtgt aaattagata aatcgtattt gtcaatcaga 4921 gtgcttttgg cgaagaatga aaatagggtt ggtactagca acgcactttg aattttgtaa 4981 tcctgaaggg atcgtaaaaa cagctcttct tcaaatctat acattaagac gactcgaaat 5041 ccacatatca aatatccgag tgtagtaaac attccaaaac cgtgatggaa tggaacaaca 5101 cttaaaatcg cagtatccgg aatgatttga ttgccaaaaa taggatctct ggcatgcgag 5161 aatctgacgc aggcagttct atgcggaagg gccacaccct taggtaaccc agtagatcca 5221 gaggagttca ttatcagtgc aattgttttg tcacgatcaa aggactctgg tacaaaatcg 5281 tattcattaa aaccgggagg tagatgagat gtgacgaacg tgtacatcga ctgaaatccc 5341 tggtaatccg ttttagaatc catgataata attttctgga ttattggtaa ttttttttgc 5401 acgttcaaaa ttttttgcaa cccctttttg gaaacaaaca ctacggtagg ctgcgaaatg 5461 ttcatactgt tgagcaattc acgttcatta taaatgtcgt tcgcgggcgc aactgcaact 5521 ccgataaata acgcgcccaa caccggcata aagaattgaa gagagttttc actgcatacg 5581 acgattctgt gatttgtatt cagcccatat cgtttcatag cttctgccaa ccgaacggac 5641 atttcgaagt attccgcgta cgtgatgttc acctcgatat gtgcatctgt aaaagcaatt 5701 gttccaggaa ccagggcgta tctcttcata gccttatgca gttgctctcc agcggttcca 5761 tcctctagcg gatagaatgg cgccgggcct ttctttatgt ttttggcgtc ttccatggtg 5821 aattc //