LOCUS       pAAV-TetOne Vector           4761 bp ds-DNA    circular SYN 13-NOV-2014
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat No. 634310, 634311
SOURCE      synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="5' ITR"
     polyA_signal    148..282
                     /label="SV40 polyA signal"
     CDS             complement(283..1029)
                     /label="Tet-On 3G"
     promoter        complement(1048..1558)
                     /label="hPGK promoter"
     misc_feature    1578..1731
                     /label="Transcription blocker"
                     /note="Transcription blocker;RNA polymerase II 
                     transcriptional pause signal from the human alpha-2 globin 
                     gene combined with a synthetic polyA signal"
     promoter        1738..2102
                     /label="TRE3GS promoter"
     misc_feature    2103..2134
                     /label="MCS"
     polyA_signal    2135..2269
                     /label="SV40 polyA signal"
     misc_feature    2279..2419
                     /label="3' ITR"
     rep_origin      complement(2837..3425)
                     /label="ori"
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3596..4456)
                     /label="AmpR"
                     /note="beta-lactamase, confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(4457..4561)
                     /label="AmpR promoter"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tagatctaac ttgtttattg cagcttataa tggttacaaa
      181 taaggcaata gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt
      241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcttacccgg ggagcatgtc
      301 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
      361 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc
      421 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
      481 ttcggctccc tgccggtcga acagctcaat tgcttgtttc agaagtgggg gcatagaatc
      541 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
      601 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
      661 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
      721 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggtatgactt
      781 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcagg agtgggtatg
      841 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
      901 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
      961 ttcgataccg actccattga gtaattccag agcagagttt atgactttgc tcttgtccag
     1021 tctagacatg gtaattcgat gatccccctg gggagagagg tcggtgattc ggtcaacgag
     1081 ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag
     1141 gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct
     1201 gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc
     1261 tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt
     1321 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
     1381 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
     1441 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
     1501 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccgt
     1561 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     1621 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     1681 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tctcgagttt
     1741 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
     1801 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
     1861 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
     1921 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
     1981 tataagcttt gcttatgtaa accagggcgc ctataaaaga gtgctgattt tttgagtaaa
     2041 cttcaattcc acaacacttt tgtcttatac caactttccg taccacttcc taccctcgta
     2101 aagaattcgc ggccgcacgc gtggatccac cggtgatcca gacatgataa gatacattga
     2161 tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg
     2221 tgatgctatt gccttatttg taaccattat aagctgcaat aaacaagttg atatcgatag
     2281 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     2341 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     2401 gcgcgcagct gcctgcaggc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     2461 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2521 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2581 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2641 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2701 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2761 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2821 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2881 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2941 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3001 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3061 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3121 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3181 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3241 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3301 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3421 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3481 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3541 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3601 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3661 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3721 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3781 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3841 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3901 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4201 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4621 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gccagctggc gaaaggggga
     4681 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     4741 acgacggcca gtgccaagct g
//
