LOCUS       pE2-Crimson-N1   4689 bp    DNA   circular            28-JAN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632554
FEATURES             Location/Qualifiers
     CDS             679..1356
                     /codon_start=1
                     /transl_table=1
                     /label=E2-Crimson
                     /note="E2-Crimson"
     CDS             2585..3379
                     /codon_start=1
                     /transl_table=1
                     /label=Kan(R)/Neo(R)
     rep_origin      3964..4607
                     /label=pUC19\ori
     promoter        1..589
                     /label=CMV\IE\Promoter
     misc_feature    672..682
                     /label=Kozak\sequence
     misc_signal     1354..1356
                     /label=Stop\codon
     misc_structure  591..671
                     /label=MCS
     misc_signal     1508..1513
                     /label=SV40\early\polyA\signal
     misc_signal     1537..1542
                     /label=SV40\early\polyA\signal
     misc_feature    complement(1605..2060)
                     /label=f1\origin\of\replication
     misc_feature    2401..2539
                     /label=SV40\ori
     misc_feature    3615..3620
                     /label=HSV\TK\polyA\signal
     misc_feature    3628..3633
                     /label=HSV\TK\polyA\signal
BASE COUNT     1113 a      1273 c      1236 g      1067 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccactag tcgccaccat ggatagcact gagaacgtca tcaagccctt catgcgcttc 
      721 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgtgggcgag 
      781 ggcaagccct acgagggcac ccagaccgcc aagctgcaag tgaccaaggg cggccccctg 
      841 cccttcgcct gggacatcct gtccccccag ttcttctacg gctccaaggc gtacatcaag 
      901 caccccgccg acatccccga ctacctcaag cagtccttcc ccgagggctt caagtgggag 
      961 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag 
     1021 gacggcaccc tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc 
     1081 gtaatgcaga agaagactct gggctgggag ccctccactg agcgcaacta cccccgcgac 
     1141 ggcgtgctga agggcgagaa ccacatggcg ctgaagctga agggcggcgg ccactacctg 
     1201 tgtgagttca agtccatcta catggccaag aagcccgtga agctgcccgg ctaccactac 
     1261 gtggactaca agctcgacat cacctcccac aacgaggact acaccgtggt ggagcagtac 
     1321 gagcgcgccg aggcccgcca ccacctgttc cagtagcggc cgcgactcta gatcataatc 
     1381 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 
     1441 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 
     1501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     1561 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa 
     1621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 
     1681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 
     1741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 
     1801 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 
     1861 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 
     1921 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 
     1981 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 
     2041 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     2101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     2161 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat 
     2221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 
     2281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 
     2341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
     2401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     2461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 
     2521 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 
     2581 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 
     2641 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 
     2701 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 
     2761 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 
     2821 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 
     2881 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 
     2941 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 
     3001 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 
     3061 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 
     3121 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 
     3181 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 
     3241 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 
     3301 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 
     3361 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 
     3421 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 
     3481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 
     3541 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 
     3601 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 
     3661 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 
     3721 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 
     3781 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 
     3841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     3901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     3961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     4021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     4081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 
     4141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     4201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     4261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     4321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     4381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     4441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     4501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     4561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     4621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     4681 gccatgcat 
//
