LOCUS       pEF1a-Tet3G   7859 bp    DNA   circular            10-JUN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631162,631167
FEATURES             Location/Qualifiers
     polyA_signal    2264..2706
                     /label=SV40\polyA\signal
     rep_origin      3074..3717
                     /label=pUC\origin
     CDS             complement(3864..4724)
                     /label=Amp(R)
     CDS             complement(6136..6930)
                     /label=Kan(R)/Neo(R)
     promoter        complement(7251..7594)
                     /label=SV40\early\Promoter
     misc_feature    53..1387
                     /label=Human\EF1a\Promoter
     misc_feature    1495..2241
                     /label=Tet-On\3G
     misc_feature    complement(4995..5529)
                     /label=SV40\polyA\signal
BASE COUNT     1866 a      2062 c      2053 g      1878 t 
ORIGIN
        1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta cagagtaatt 
       61 catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact 
      121 aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac 
      181 tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg 
      241 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg 
      301 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 
      361 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 
      421 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt 
      481 tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga 
      541 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc 
      601 ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg 
      661 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg 
      721 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct 
      781 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg 
      841 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc 
      901 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg 
      961 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg 
     1021 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc 
     1081 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga 
     1141 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt 
     1201 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga 
     1261 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg 
     1321 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt 
     1381 gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 
     1441 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccatgtct 
     1501 agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg agtcggtatc 
     1561 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac 
     1621 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg 
     1681 catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac 
     1741 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc 
     1801 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag 
     1861 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg 
     1921 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc 
     1981 accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc 
     2041 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag 
     2101 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc 
     2161 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac 
     2221 cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata cattgatgag 
     2281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 
     2341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 
     2401 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 
     2461 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca 
     2521 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc 
     2581 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc 
     2641 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg 
     2701 aagtatccag ctcgaccaag cttggcgaga ttttcaggag ctaaggaagc taaaatggag 
     2761 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 
     2821 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct gcattaatga 
     2881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     2941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     3001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     3061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     3121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     3181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     3241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     3301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     3361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     3421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     3481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     3541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     3601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     3661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     3721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     3781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     3841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     3901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     3961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     4021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     4081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     4141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     4201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     4261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     4321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     4381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     4441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
     4501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     4561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     4621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     4681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     4741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     4801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     4861 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     4921 cgtcttcact cgaggtcgag ggatccagac atgataagat acattgatga gtttggacaa 
     4981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 
     5041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 
     5101 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 
     5161 tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa 
     5221 cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag 
     5281 acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc 
     5341 tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt 
     5401 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca 
     5461 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg 
     5521 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg 
     5581 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat 
     5641 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta 
     5701 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca 
     5761 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc 
     5821 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga 
     5881 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc 
     5941 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct 
     6001 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg 
     6061 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga 
     6121 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga 
     6181 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 
     6241 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 
     6301 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 
     6361 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa 
     6421 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 
     6481 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca 
     6541 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 
     6601 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 
     6661 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 
     6721 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt 
     6781 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 
     6841 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 
     6901 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc 
     6961 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt 
     7021 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt 
     7081 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct 
     7141 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca 
     7201 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc 
     7261 tgagtgcttg cggcagcgtg aagctagctt tttgcaaaag cctaggcctc caaaaaagcc 
     7321 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 
     7381 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 
     7441 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 
     7501 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 
     7561 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 
     7621 attccacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     7681 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     7741 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 
     7801 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcacct 
//
