miRNA purification & isolation kit—NucleoSpin miRNA
NucleoSpin miRNA is suitable for the simultaneous isolation and purification of small RNA (<200 nt; e.g., miRNA, pre-miRNA, tRNA, 5S RNA), large RNA (>200 nt; e.g., mRNA, 18S rRNA, 28S rRNA, pre-miRNA), and protein in three separate fractions from a large variety of sample materials.
NucleoSpin miRNA is suitable for the simultaneous isolation and purification of small RNA (<200 nt; e.g., miRNA, pre-miRNA, tRNA, 5S RNA), large RNA (>200 nt; e.g., mRNA, 18S rRNA, 28S rRNA, pre-miRNA), and protein in three separate fractions from a large variety of sample materials.
The precipitated protein can easily be dissolved in Laemmli buffer and used for SDS-PAGE, Western Blot analysis, and protein quantification with the Macherey-Nagel Protein Quantification Assay. The eluted RNA and miRNA are ready-to-use for all standard downstream applications, such as RT-PCR, Northern blotting, and chip hybridization.
Overview
- Effective isolation of miRNA, other RNA, and total RNA from a variety of samples
- Effective purification of miRNA and total RNA in two fractions
- Effective purification of miRNA and total RNA in one fraction
- Ideal for isolation and purification of miRNA, siRNA, shRNA, snRNA, and other small RNAs
- Isolate total protein fraction
- Choice of lysis conditions, with or without phenol/chloroform
- Complete kit includes all material
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | <107 cultured cells <30 mg human/animal tissue <50 mg plant tissue <150 µl reaction mixture |
| Fragment size – miRNA | 18–200 bases |
| Fragment size – Large RNA | >200 bases |
| Typical yield (from 107 HeLa Cells) |
10 µg small RNA 95 µg large RNA |
| Binding capacity | 200 µg |
| Elution volume | 30–100 µl |
| Preparation time | <45 min (6 preps human/animal tissue; miRNA and large RNA) <35 min (6 preps human/animal tissue; miRNA only) |
Applications
- Parallel isolation and purification of miRNA and large RNA from human/animal tissue and cultured cells
- Parallel isolation and purification of miRNA and large RNA from plant tissue
- Parallel isolation and purification of miRNA and large RNA in combination with phenol/chloroform (e.g., Trizol) lysis
- Isolation and purification of miRNA, large RNA, and protein from the same sample
- Purification of siRNA and large dsRNA from DICER reactions
RNA is suitable for:
- Northern blot analysis
- Real-time RT-PCR
- Microarray analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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