LOCUS Exported 7804 bp ds-DNA circular 05-JUL-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. Nos. 630466, 630489, 630498, 630499 SOURCE synthetic DNA construct FEATURES Location/Qualifiers source 1..7804 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 456..1160 /gene="S. cerevisiae ADH1" CDS 1188..1628 /label=GAL4 DNA binding domain promoter 1639..1657 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1677..1706 /label=Myc tag misc_feature 1740..2244 /label=Human Lamin A/C terminator 2265..2312 /label=T7 terminator terminator 2339..2526 /gene="S. cerevisiae ADH1" /label=ADH1 terminator rep_origin 2952..3540 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" polyA_signal 3869..3916 /label=HSV TK poly(A) signal CDS complement(4148..4942) /label=NeoR/KanR promoter complement(4943..5047) /label=AmpR promoter rep_origin 5074..6416 /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 6675..6956 /label=TRP1 promoter CDS 6957..7631 /label=TRP1 rep_origin complement(join(7761..7804,1..385)) /label=f1 ori ORIGIN 1 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 61 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag 421 ggcgatcggt gcgggcctct tcgctattac gccagatcct tttgttgttt ccgggtgtac 481 aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct ataatacctt 541 cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac agataccaga 601 caagacataa tgggctaaac aagactacac caattacact gcctcattga tggtggtaca 661 taacgaacta atactgtagc cctagacttg atagccatca tcatatcgaa gtttcactac 721 cctttttcca tttgccatct attgaagtaa taataggcgc atgcaacttc ttttcttttt 781 ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa aaaaatgatg 841 gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc gttgttccag 901 agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt cacgcacact 961 actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt gaaataaaaa 1021 aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc ttttgtttcc 1081 tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta 1141 taccaagcat acaatcaact ccaagcttga agcaagcctc ctgaaagatg aagctactgt 1201 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa 1261 aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca 1321 aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg 1381 aacagctatt tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt 1441 ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag 1501 atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc 1561 atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga 1621 ctgtatcgcc ggaatttgta atacgactca ctatagggcg agccgccatc atggaggagc 1681 agaagctgat ctcagaggag gacctgcata tggccatgga ggccgaattc ccggggatct 1741 ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag gccgagctcg 1801 gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg cagctggagc 1861 tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag aaggagggtg 1921 acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac tccaaggagg 1981 ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg catgatctgc 2041 ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa cttcaggatg 2101 agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag gaactggact 2161 tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat gagacccgac 2221 tggtggagat tgacaggatc cgtcgacctg cagcggccgc ataactagca taaccccttg 2281 gggcctctaa acgggtcttg aggggttttt tgcgcgcttg cagccaagct aattccgggc 2341 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata 2401 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt 2461 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc 2521 taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 2581 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 2641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 2701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 2761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 3001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 3061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 3121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 3181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 3241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 3421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3661 tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc 3721 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa 3781 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc 3841 cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc 3901 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca 3961 gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc 4021 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg 4081 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga 4141 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag 4201 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa 4261 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt 4321 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat 4381 gagtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg 4441 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca 4501 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg 4561 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag 4621 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc 4681 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg 4741 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa 4801 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg 4861 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt 4921 gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg 4981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5041 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta 5101 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt 5161 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt 5221 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca 5281 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt 5341 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga 5401 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt 5461 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag 5521 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag 5581 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga 5641 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt 5701 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta 5761 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa 5821 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat 5881 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg 5941 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg 6001 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa 6061 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca 6121 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg 6181 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat 6241 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc 6301 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac 6361 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac 6421 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 6481 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 6541 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 6601 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 6661 gtgcaccata gatcaacgac attactatat atataatata ggaagcattt aatagaacag 6721 catcgtaata tatgtgtact ttgcagttat gacgccagat ggcagtagtg gaagatattc 6781 tttattgaaa aatagcttgt caccttacgt acaatcttga tccggagctt ttcttttttt 6841 gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt 6901 ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt 6961 ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca 7021 cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc 7081 ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag 7141 catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta 7201 aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag 7261 atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat 7321 ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc 7381 ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg 7441 actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga 7501 cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg 7561 gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg 7621 ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat 7681 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt 7741 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 7801 ccaa //