LOCUS pGBT9 Vector 5523 bp ds-DNA circular 05-JUN-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. No. 630439 SOURCE synthetic DNA construct FEATURES Location/Qualifiers source 1..5523 promoter 10..406 /label=ADH1 promoter CDS 434..874 /label=GAL4 DNA binding domain terminator 921..1108 /label=ADH1 terminator CDS complement(1161..1835) /label=TRP1 promoter complement(1836..1937) /label=TRP1 promoter rep_origin 2353..2941 /label=ori CDS complement(3112..3972) /label=Amp(R) promoter complement(3973..4077) /label=AmpR promoter rep_origin 4359..5523 /label=2u ori /note="yeast 2u plasmid origin of replication" ORIGIN 1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 61 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac 121 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact 181 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt 241 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga 301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca 421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact 481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg 541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga 601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga 661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt 721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac 781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag 841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc 901 tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag 961 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat 1021 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg 1081 tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat 1141 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta 1201 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt 1261 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag 1321 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca 1381 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct 1441 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg 1501 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg 1561 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag 1621 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca 1681 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca 1741 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat 1801 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta 1861 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt 1921 cttttttcga ccgaattaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 1981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 2041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 2101 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 2161 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2221 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 2281 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2341 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2401 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 2461 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 2521 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 2581 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 2641 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 2701 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 2761 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 2821 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 2881 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 2941 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3001 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3061 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3121 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3181 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3241 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 3301 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 3361 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 3421 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 3481 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 3541 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 3601 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 3661 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 3721 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 3781 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 3841 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 3901 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 3961 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 4021 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4081 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 4141 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 4201 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 4261 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 4321 tgcggcatca gagcagattg tactgagagt gcaccataac gcatttaagc ataaacacgc 4381 actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg 4441 tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga 4501 aacgctttga agttcctatt ccgaagttcc tattctctag ctagaaagta taggaacttc 4561 agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca 4621 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa 4681 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc 4741 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta 4801 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga 4861 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat 4921 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc 4981 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt 5041 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag 5101 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga 5161 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat 5221 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa 5281 atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa 5341 aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg 5401 gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt 5461 ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc 5521 gtt //