LOCUS pAAV-Guide-it-Up Vector 7117 bp ds-DNA circular SYN 10-MAR-2015 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat No. 632608, 632609, 632610 FEATURES Location/Qualifiers misc_feature 1..141 /label="5' ITR" enhancer 210..513 /label="CMV enhancer" /note="human cytomegalovirus (CMV) immediate early enhancer" promoter 514..717 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 748..3853 /label="Cas9-Up" misc_feature 3860..4000 /label="3' ITR" promoter 4976..5080 /label="AmpR promoter" CDS 5081..5941 /label="AmpR" /note="beta-lactamase, confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 6112..6700 /direction=RIGHT /label="ori" /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tatcgatatc aagctttaat agtaatcaat tacggggtca 181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 721 ttagtggata tccttaaggc cgccaccatg gacaagaagt acagcatcgg cctggacatc 781 ggtaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag 841 ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg 901 ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc 961 tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc 1021 aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag 1081 aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag 1141 taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg 1201 cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt cctgatcgag 1261 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc 1321 tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc caaggccatc 1381 ctgagcgccc gcctgagcaa gagccgccgc ctggagaacc tgatcgccca gctgcccggc 1441 gagaagaaga acggcctgtt cggcaacctg atcgccctga gcctgggcct gacccccaac 1501 ttcaagagca acttcgacct ggccgaggac gccaagctgc agctgagcaa ggacacctac 1561 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgttcctg 1621 gccgccaaga acctgagcga cgccatcctg ctgagcgaca tcctgcgcgt gaacaccgag 1681 atcaccaagg cccccctgag cgccagcatg atcaagcgct acgacgagca ccaccaggac 1741 ctgaccctgc tgaaggccct ggtgcgccag cagctgcccg agaagtacaa ggagatcttc 1801 ttcgaccaga gcaagaacgg ctacgccggc tacatcgacg gcggcgccag ccaggaggag 1861 ttctacaagt tcatcaagcc catcctggag aagatggacg gcaccgagga gctgctggtg 1921 aagctgaacc gcgaggacct gctgcgcaag cagcgcacct tcgacaacgg cagcatcccc 1981 caccagatcc acctgggcga gctgcacgcc atcctgcgcc gccaggagga cttctacccc 2041 ttcctgaagg acaaccgcga gaagatcgag aagatcctga ccttccgcat cccctactac 2101 gtgggccccc tggcccgcgg caacagccgc ttcgcctgga tgacccgcaa gagcgaggag 2161 accatcaccc cctggaactt cgaggaggtg gtggacaagg gcgccagcgc ccagagcttc 2221 atcgagcgca tgaccaactt cgacaagaac ctgcccaacg agaaggtgct gcccaagcac 2281 agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaggtgaa gtacgtgacc 2341 gagggcatgc gcaagcccgc cttcctgagc ggcgagcaga agaaggccat cgtggacctg 2401 ctgttcaaga ccaaccgcaa ggtgaccgtg aagcagctga aggaggacta cttcaagaag 2461 atcgagtgct tcgacagcgt ggagatcagc ggcgtggagg accgcttcaa cgccagcctg 2521 ggcacctacc acgacctgct gaagatcatc aaggacaagg acttcctgga caacgaggag 2581 aacgaggaca tcctggagga catcgtgctg accctgaccc tgttcgagga ccgcgagatg 2641 atcgaggagc gcctgaagac ctacgcccac ctgttcgacg acaaggtgat gaagcagctg 2701 aagcgccgcc gctacaccgg ctggggccgc ctgagccgca agcttatcaa cggcatccgc 2761 gacaagcaga gcggcaagac catcctggac ttcctgaaga gcgacggctt cgccaaccgc 2821 aacttcatgc agctgatcca cgacgacagc ctgaccttca aggaggacat ccagaaggcc 2881 caggtgagcg gccagggcga cagcctgcac gagcacatcg ccaacctggc cggcagcccc 2941 gccatcaaga agggcatcct gcagaccgtg aaggtggtgg acgagctggt gaaggtgatg 3001 ggccgccaca agcccgagaa catcgtgatc gagatggccc gcgagaacca gaccacccag 3061 aagggccaga agaacagccg cgagcgcatg aagcgcatcg aggagggcat caaggagctg 3121 ggcagccaga tcctgaagga gcaccccgtg gagaacaccc agctgcagaa cgagaagctg 3181 tacctgtact acctgcagaa cggccgcgac atgtacgtgg accaggagct ggacatcaac 3241 cgcctgagcg actacgacgt ggaccacatc gtgccccaga gcttcctgaa ggacgacagc 3301 atcgacaaca aggtgctgac ccgcagcgac aagaaccgcg gcaagagcga caacgtgccc 3361 agcgaggagg tggtgaagaa gatgaagaac tactggcgcc agctgctgaa cgccaagctg 3421 atcacccagc gcaagttcga caacctgacc aaggccgagc gcggcggcct gagcgagctg 3481 gacaaggccg gcttcatcaa gcgccagctg gtggagaccc gccagatcac caagcacgtg 3541 gcccagatcc tggacagccg catgaacacc aagtacgacg agaacgacaa gctgatccgc 3601 gaggtgaagg tgatcaccct gaagagcaag ctggtgagcg acttccgcaa ggacttccag 3661 ttctacaagg tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc 3721 gtggtgggca ccgccctgat caagaagtac cccaagctgg agagcgagtt cgtgtacggc 3781 gactacaagg tgtacgacgt gcgcaagatg atcgccaaga gcgagcagga gatcggcaag 3841 gccaccgcca agtagatcta ggaaccccta gtgatggagt tggccactcc ctctctgcgc 3901 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 3961 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg cagcttggca ctggccgtcg 4021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 4081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 4141 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt 4201 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt 4261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 4321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 4381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 4441 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 4501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 4561 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc 4621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 4681 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4741 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4801 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4861 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 4921 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 4981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 5041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 5101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 5161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 5221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 5281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 5341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 5401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 5461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 5521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 5581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 5641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 5701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 5761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 5821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 5881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 5941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 6001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 6061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 6121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 6181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 6241 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 6301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 6361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 6421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 6481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 6541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 6601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 6661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 6721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 6781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 6841 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 6901 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 6961 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 7021 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 7081 tcacacagga aacagctatg accatgatta cgaattg //