LOCUS pAcGFP1-F 4806 bp DNA circular 7-AUG-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 632511 FEATURES Location/Qualifiers misc_feature 1345..1407 /label=c-Ha-Ras\farnesylation promoter 1..589 /label=CMV\IE\Promoter rep_origin 2518..2656 /label=SV40\ori CDS 2702..3496 /label=Kan(R)/Neo(R) /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157" rep_origin 4081..4724 /label=pUC\ori rep_origin complement(1722..2177) /label=f1_ori CDS 613..1329 /label=AcGFP1 polyA_signal 1625..1630 /label=SV40\early\polyA\signal polyA_signal 1654..1659 /label=SV40\early\polyA\signal polyA_signal 3732..3737 /label=HSV\TK\Poly\A\signal polyA_signal 3745..3750 /label=HSV\TK\Poly\A\signal misc_feature 606..616 /label=Kozak\consensus\translation\initiation\site misc_feature 2574..2580 /label=SV40\early\promoter\element BASE COUNT 1156 a 1291 c 1259 g 1100 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 661 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 781 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 841 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 901 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 961 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 1021 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 1081 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 1141 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 1201 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 1261 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 1321 ctgtacaagt ccggactcag atctaagctg aaccctcctg atgagagtgg ccccggctgc 1381 atgagctgca agtgtgtgct ctcctgagga tccagatctc gagctcaagc ttcgaattct 1441 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc 1501 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 1561 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 1621 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 1681 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 1741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 1801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 1861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 1921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 1981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2161 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2221 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2281 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 2341 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2401 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 2461 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 2521 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 2581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 2641 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 2701 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 2761 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 2821 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 2881 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 2941 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3001 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3061 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3121 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3181 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 3241 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3301 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3361 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3421 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3481 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 3541 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3601 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3661 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 3721 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 3781 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 3841 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 3901 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 3961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 4801 atgcat //