LOCUS pLVX-TRE3G-Luc Control Vector 9404 bp ds-DNA circular 05-JUN-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. No.631191, 631193, 631187, 631189, 631191, 631193, 631360, 631361, 631362, 631187, 631189, 631349, 631350, 631351, 631352, 631353, 631354, 631355, 631356, 631357, 635087 SOURCE synthetic DNA construct FEATURES Location/Qualifiers source 1..9404 /organism="synthetic DNA construct" LTR 1..634 /label=5' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi misc_feature 1303..1536 /label=RRE misc_feature 2028..2143 /label=cPPT/CTS promoter 2206..2555 /label=TRE3GV promoter CDS 2565..4217 /label=luciferase promoter 4237..4736 /label=PGK promoter CDS 4757..5356 /label=PuroR misc_feature 5373..5961 /label=WPRE LTR 6168..6801 /label=3' LTR rep_origin 7331..7919 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8090..8950) /label=AmpR ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 2521 ctggagcaat tccacaacac ttttgtctta tacttggatc caccatggaa gacgccaaaa 2581 acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct ggagagcaac 2641 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 2701 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag 2761 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 2821 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 2881 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag 2941 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa 3001 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 3061 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct 3121 ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta 3181 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta 3241 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 3301 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 3361 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt 3421 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt 3481 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag 3541 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta 3601 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 3661 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 3721 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg 3781 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 3841 gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag 3901 gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg 3961 acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 4021 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 4081 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 4141 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 4201 gaaagtccaa attgtaaaat acgcgtgaat tctaccgggt aggggaggcg cttttcccaa 4261 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 4321 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 4381 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 4441 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 4501 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 4561 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 4621 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 4681 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct 4741 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 5341 caagcccggt gcctgacggg cgcgtctgga acaatcaacc tctggattac aaaatttgtg 5401 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 5461 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 5521 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 5581 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 5641 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 5701 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 5761 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 5821 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 5881 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 5941 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg 6001 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 6061 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 6121 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat 6181 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 6241 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg 6301 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa 6361 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt 6421 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga 6481 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc 6541 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc 6601 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6781 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 6841 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga 6901 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 7021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9061 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 9241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 9301 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 9361 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt 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