LOCUS pLVX_TRE3G_mCherry 9066 bp ds-DNA circular 05-JUN-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. No. 631349, 631352, 631355, 631360 SOURCE synthetic DNA construct FEATURES Location/Qualifiers source 1..9066 LTR 1..634 /label=5' LTR misc_feature 681..806 /label=HIV-1 Psi misc_feature 1303..1536 /label=RRE misc_feature 2028..2143 /label=cPPT/CTS promoter 2206..2555 /label=TRE3GV promoter CDS 2564..3274 /label=mCherry /note="mammalian codon-optimized" misc_feature 3290..3863 /label=IRES promoter 3899..4398 /label=PGK promoter CDS 4419..5018 /label=PuroR misc_feature 5035..5623 /label=WPRE LTR 5830..6463 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" rep_origin 6993..7581 /label=ori CDS complement(7752..8612) /label=AmpR promoter complement(8613..8717) /label=AmpR promoter polyA_signal 8765..8899 /label=SV40 poly(A) signal ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggtga gcaagggcga 2581 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 2641 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2701 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2761 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2821 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2881 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 2941 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 3001 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 3061 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 3121 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 3181 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 3241 ctccaccggc ggcatggacg agctgtacaa gtagggccgc cccggccggc ccctctccct 3301 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 3361 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 3421 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 3481 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 3541 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 3601 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 3661 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 3721 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta 3781 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 3841 tcctttgaaa aacacgatga taatatggcc cccgggacgc gtcatatgga attctaccgg 3901 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 3961 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 4021 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 4081 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 4141 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 4201 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 4261 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 4321 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 4381 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg 4441 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 4501 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 4561 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 4621 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 4681 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 4741 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 4801 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 4861 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 4921 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 4981 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg ggcgcgtctg gaacaatcaa 5041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5401 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 5461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 5581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca 5641 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 5701 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 5761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5821 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 5881 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 5941 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 6001 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 6061 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 6121 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 6181 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag 6241 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac 6301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6481 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga 6541 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 6601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6721 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 6781 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 6841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6961 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 7441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 7681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 7741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 7801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 7861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 7921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 7981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 8461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 8521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 8581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 8641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 8701 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 8761 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8881 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 8941 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga 9001 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 9061 agtagt //