LOCUS pPAmCherry-N1 4716 bp DNA circular SYN 21-JUL-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No.632584 FEATURES Location/Qualifiers source 1..4716 /organism="recombinant plasmid" /mol_type="other DNA" promoter 1..589 /label=CMV\promoter /note="color: #66cc99" TATA_signal 554..560 /label=CMV\TATA\box /note="color: #0000ff" misc_feature 591..665 /label=MCS /note="color: #66cc99" CDS 672..1379 /label=PAmCherry1 /note="color: #ff9966" misc_feature 1380..1382 /label=stop /note="color: #3366cc" rep_origin complement(1632..2087) /label=f1\ori /note="color: #ff9966" rep_origin 2428..2566 /label=SV40\ori /note="color: #ff9966" CDS 2612..3406 /label=Kan(R)/Neo(R) /note="color: #ff9966" rep_origin 3991..4634 /label=pUC\ori /note="color: #ff9966" misc_feature 666..675 /label=Kozak\consensus\sequence promoter 2261..2529 /label=SV40\e\promoter polyA_signal 3642..3660 /label=HSV\TK\polyA\signal polyA_signal 1535..1569 /label=SV40\poly\A BASE COUNT 1130 a 1262 c 1260 g 1064 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccgccac catggtgagc aagggcgagg aggataacat ggccatcatt aaggagttca 721 tgcgcttcaa ggtgcacatg gaggggtccg tgaacggcca cgtgttcgag atcgagggcg 781 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 841 gccccctgcc cttcacctgg gacatcctgt cccctcaatt catgtacggc tccaatgcct 901 acgtgaagca ccccgccgac atccccgact actttaagct gtccttcccc gagggcttca 961 agtgggagcg cgtgatgaaa ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 1021 ccctgcagga cggtgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1081 acggccccgt aatgcagaag aagaccatgg gctgggaggc cctctccgag cggatgtacc 1141 ccgaggacgg cgccctgaag ggcgaggtca agccgagagt gaagctgaag gacggcggcc 1201 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1261 cctacaacgt caaccgcaag ttggacatca cctcacacaa cgaggactac accatcgtgg 1321 aacagtacga acgtgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 1381 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 1441 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 1501 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 1561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 1621 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 1741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 1801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 1861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 1921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 1981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 2101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 2221 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2281 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2401 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 2521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 2581 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2641 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2701 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2761 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 2821 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2881 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 2941 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3001 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3061 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3121 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3181 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 3241 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 3301 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 3361 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3421 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 3481 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3541 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 3601 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3661 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 3721 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 3781 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 3841 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 3901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 3961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 4441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 4561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 4621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 4681 cctgattctg tggataaccg tattaccgcc atgcat //