LOCUS pRetroX-Tet3G 8136 bp DNA circular 31-MAY-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT 631192, 631188. FEATURES Location/Qualifiers LTR 1..728 /label=5'LTR(CMV/MSV) misc_feature 759..1568 /label=Packaging\signal promoter 1601..2132 /label=pCMV misc_feature 3042..3615 /label=IRES LTR 4840..5265 /label=3'\LTR rep_origin 5765..5830 /label=SV40\ori promoter 5544..5811 /label=pSV40 rep_origin 6152..6152 /label=pUC\ori rep_origin 6911..7771 /label=Amp(R) CDS 2290..3036 /label=Tet-On\3G misc_feature 3631..4425 /label=Neo(R) BASE COUNT 1886 a 2214 c 2093 g 1943 t ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga 2281 tccgccacca tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc 2341 aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag 2401 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc 2461 gagatgctgg acaggcatca tacccactcc tgccccctgg aaggcgagtc atggcaagac 2521 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct 2581 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc 2641 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg 2701 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa 2761 agagagacac ctaccaccga ttctatgccc ccacttctga aacaagcaat tgagctgttc 2821 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg 2881 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta 2941 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct 3001 cttgacgatt ttgaccttga catgctcccc gggtaagaat tccgcccctc tccctccccc 3061 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg 3121 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc 3181 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg 3241 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg 3301 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 3361 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 3421 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 3481 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 3541 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 3601 gaaaaacacg atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt 3661 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3721 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3781 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 3841 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3901 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3961 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 4021 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 4081 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 4141 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 4201 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 4261 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 4321 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 4381 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga 4441 gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca 4501 tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc 4561 tgcgcgacgg cgagctgctc accacccact cgagggctgc agcgctgcag aggccgagtg 4621 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag 4681 ccagtgtaga gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc 4741 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag 4801 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt 4861 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg 4921 agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac 4981 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 5041 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 5101 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc 5161 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 5221 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc 5281 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc 5341 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 5401 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 5461 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc 5521 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 5581 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 5701 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 5761 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 5821 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact 5881 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 5941 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 6001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7201 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg 7261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7501 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 7561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 7921 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag 7981 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct 8041 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa 8101 ttacggggtc attagttcat agcccatata tggagt //