LOCUS pRetroX-TRE3G-Luc Control 8233 bp DNA circular 25-MAY-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT 631192, 631194, 631188, 631190 FEATURES Location/Qualifiers LTR 1..728 /label=5'\LTR(CMV/MSV) misc_feature 759..1568 /label=Packaging\Signal CDS 1967..3619 /label=Luciferace promoter 3635..4138 /label=pPGK CDS 4159..4758 /label=Puro(R) LTR 4939..5364 /label=3'\LTR polyA_signal 5266..5271 /label=SV40\polyA promoter 5644..5911 /label=pSV40 rep_origin 5865..5930 /label=SV40\ori rep_origin 6221..6864 /label=pUC\ori misc_feature complement(7011..7871) /label=Amp(R) misc_feature 1611..1957 /label=pTRE3G BASE COUNT 1958 a 2210 c 2093 g 1972 t ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaagat ctcgatgagg ccctttcgtc ttcactcgag tttactccct 1621 atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag 1681 actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga 1741 gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct 1801 atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga acgtatgtcg 1861 aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc 1921 gcctggagca attccacaac acttttgtct tatacttgga tccaccatgg aagacgccaa 1981 aaacataaag aaaggcccgg cgccattcta tccgctagag gatggaaccg ctggagagca 2041 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc 2101 acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga 2161 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc 2221 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc 2281 gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt 2341 agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat 2401 aatccagaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta 2461 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc 2521 ctttgatcgt gacaaaacaa ttgcactgat aatgaactcc tctggatcta ctgggttacc 2581 taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc 2641 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca 2701 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat 2761 gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa ttcaaagtgc 2821 gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg acaaatacga 2881 tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga 2941 agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc tcactgagac 3001 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt 3061 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa 3121 tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg taaacaatcc 3181 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta 3241 ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa 3301 aggataccag gtggcccccg ctgaattgga gtcgatattg ttacaacacc ccaacatctt 3361 cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt 3421 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca 3481 agtaacaacc gccaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg 3541 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg 3601 cggaaagtcc aaattgtaaa atacgcgtga attctaccgg gtaggggagg cgcttttccc 3661 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 3721 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 3781 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 3841 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 3901 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 3961 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 4021 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 4081 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 4141 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 4741 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca 4801 cgaccccatg catcggcgtc tcgagatatc agtggtccag gctctagttt tgactcaaca 4861 atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag 4921 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt 4981 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag 5041 gtcaggaaca gatggaacag ggtcgaccct agagaaccat cagatgtttc cagggtgccc 5101 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 5161 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 5221 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 5281 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 5341 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag 5401 ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg 5461 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 5521 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 5581 catgacccag tcacgtagcg atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt 5641 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 5701 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 5761 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 5821 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 5881 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 5941 aggcctaggc ttttgcaaaa agcttactgg cttaactatg cggcatcaga gcagattgta 6001 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 6061 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 6121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 6181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 6241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 6301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 6361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 6421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 6481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 6541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 6601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6661 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 6721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 7021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 7081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 7141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 7201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 7261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 7321 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 7381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 7441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 7501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 7561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 7621 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 7681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 7741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 7801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 7861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 7921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 7981 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 8041 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg 8101 ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt accgccatgt 8161 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 8221 ccatatatgg agt //