NucleoSpin Soil (50) 50 preps for the isolation of DNA from soil and sediment - NucleoSpin Soil Columns, MN bead tubes, NucleoSpin Inhibitor Removal Columns, Collection Tubes, buffers

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Pack of fifty 2-ml tubes containing ceramic beads for sample homogenization. Use in combination with the MN Bead Tube Holder (Cat. # 740469) and NucleoSpin Soil (e.g., Cat. # 740780.50) or Nucleospin Stool (Cat. # 740472.50), miniprep kits for isolation of total DNA from soil, sediments, or stool samples.