rLV.EF1.AcGFP1-Actin-9
Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines
Overview of the process for generating high-titer lentiviral particles
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method
Subcellular staining of actin using recombinant lentivirus to deliver and express fusions of actin to mCherry (Cat. # 0020VCT rLV.EF1.mCherry-Actin-9) and AcGFP1 (Cat. # 0013VCT rLV.EF1.AcGFP1-Actin-9), respectively
Not all titers are the same! Comparison of cells transduced with 100 M
Map schematics of expression cassettes contained in particles for labeling subcellular compartments (e.g. actin, tubulin, mitochondria, etc.) with AcGFP1 fluorescent protein
What is a functional titer? Most vendors of ready-made viral particles determine their titers using a simple particle count
Achieve 100% transduction efficiency even in cell types that are typically hard to infect
