This marker consists of ten double-stranded DNA fragments ranging from 1 kb to 10 kb in multiples of 1 kb. The 5-kb fragment is brighter than the other fragments and serves as a visible reference indicator; all other fragments appear with equal intensity on the gel. 

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg²⁺ plus buffer and dNTP mix are supplied with the polymerase.

Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes.

Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.