EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions.

EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions. Cat. # R100B contains 2 of Cat. # R100A. Please refer to Cat. # R100A for complete product documentation and resources.

Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify.

Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify. Cat. # R110B contains 4 of Cat. # R110A. Please refer to Cat. # R110A for complete product documentation and resources.