FPLC purification of HAT-tagged protein with TALON Superflow

pHAT10/11/12 and pHAT20 Vector maps

Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags

Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions