A monoclonal antibody for the detection of Discosoma sp. red fluorescent protein (DsRed). This antibody recognizes denatured forms of wild-type DsRed and its variants, including DsRed1, DsRed2, and DsRed1-E5 (the Fluorescent Timer). The antibody binds DsRed1 and DsRed2 even when these proteins are expressed as fusions to other proteins. Both N- and C-terminal fusions are recognized. The Living Colors DsRed Monoclonal Antibody is recommended for Western blotting applications only.

The Living Colors DsRed Polyclonal Antibody has been raised against DsRed-Express, a variant of Discosoma sp. red fluorescent protein. This antibody recognizes DsRed-Express, DsRed-Express2, DsRed-Monomer, mCherry, DsRed2, E2-Crimson, tdTomato, mStrawberry, and mBanana, and both N- and C-terminal fusion proteins containing these fluorescent proteins in mammalian cell lysates.

pLVX-EF1α-IRES-mCherry is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector contains an internal ribosomal entry site (IRES) that allows a gene-of-interest and the red fluorescent protein mCherry to be simultaneously coexpressed from a single mRNA transcript. Expression of the transcript is driven by the constitutively active human elongation factor 1 alpha (EF1α) promoter.

Xfect is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. Transfections can be carried out entirely in the presence of serum.

NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection.