The Living Colors mCherry Monoclonal Antibody is Protein A-purified and was raised against mCherry. It recognizes mCherry, mOrange, mOrange2, mPlum, and mStrawberry, as well as C- and N-terminal fusions containing these fluorescent proteins.

The Living Colors DsRed Polyclonal Antibody has been raised against DsRed-Express, a variant of Discosoma sp. red fluorescent protein. This antibody recognizes DsRed-Express, DsRed-Express2, DsRed-Monomer, mCherry, DsRed2, E2-Crimson, tdTomato, mStrawberry, and mBanana, and both N- and C-terminal fusion proteins containing these fluorescent proteins in mammalian cell lysates.

The Mir-X Inducible miRNA System (Red) is a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright red fluorescent protein marker, mCherry, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the mCherry/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-mCherry/miRNA vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.

The Mir-X Inducible miRNA System (Green) contains a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright green fluorescent protein marker, ZsGreen1, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the ZsGreen1/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-ZsGreen1 Vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.

This 200 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 600 rxn kit see Cat. # 638316.