A monoclonal antibody for the detection of Discosoma sp. red fluorescent protein (DsRed). This antibody recognizes denatured forms of wild-type DsRed and its variants, including DsRed1, DsRed2, and DsRed1-E5 (the Fluorescent Timer). The antibody binds DsRed1 and DsRed2 even when these proteins are expressed as fusions to other proteins. Both N- and C-terminal fusions are recognized. The Living Colors DsRed Monoclonal Antibody is recommended for Western blotting applications only.

The Living Colors mCherry Monoclonal Antibody is Protein A-purified and was raised against mCherry. It recognizes mCherry, mOrange, mOrange2, mPlum, and mStrawberry, as well as C- and N-terminal fusions containing these fluorescent proteins.

This Retro-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of MMLV-based retroviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (C_t) of an appropriate dilution of purified viral RNA to sample curve C_t values that are derived from a calibrated, serially diluted control viral RNA template.

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used.

RetroNectin reagent is a 63-kD fragment of recombinant human fibronectin fragment (also referred to as rFN-CH-296) that enhances the efficiency of lentiviral- and retroviral-mediated gene transduction. This is particularly important for hematopoietic cells and other hard-to-transfect cell types. When coated on the surface of containers such as culture dishes, petri dishes, flasks or bags, RetroNectin significantly enhances virus-mediated gene transduction into mammalian cells. This enhanced transduction is thought to result from the colocalization of virus particles and target cells on the molecules of RetroNectin. Viral particles bind RetroNectin via interaction with heparin-binding domain II, and target cells bind mainly through the interaction of the cell surface integrin receptor VLA-4 with the fibronectin CS1 site.

The Living Colors DsRed Polyclonal Antibody has been raised against DsRed-Express, a variant of Discosoma sp. red fluorescent protein. This antibody recognizes DsRed-Express, DsRed-Express2, DsRed-Monomer, mCherry, DsRed2, E2-Crimson, tdTomato, mStrawberry, and mBanana, and both N- and C-terminal fusion proteins containing these fluorescent proteins in mammalian cell lysates.

The Living Colors Anti-RCFP Polyclonal Pan Antibody was raised in a rabbit against recombinant full-length Zoanthus sp. yellow fluorescent protein (ZsYellow). This pan antibody recognizes denatured forms of AmCyan, ZsGreen, ZsYellow, DsRed2, DsRed-Express, HcRed, AsRed, as well as fusion proteins containing these RCFP variants in bacterial and mammalian cell lysates.