SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 384 reactions.

SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 24 reactions.

SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 96 reactions.

The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis.

The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis.

The SMARTer-Seq Magnetic Separator - PCR Strip is designed especially for separation of magnetic beads (like AMPure XP beads; Beckman Coulter), from solution. This stand holds up to 24 0.2-ml PCR tubes in two rows, and is designed for capture of magnetic beads in 10–20 minutes. It is ideally suited for magnetic bead-based DNA purification and size selection techniques as used with most of our products for next-generation sequencing.