A convenient 2X PCR master mix which consist of Takara Taq HS polymerase, optimized reaction buffer, and dNTPs. Takara Taq HS polymerase is an antibody-mediated hot-start version of TaKaRa Taq DNA Polymerase, which is a recombinant version of full-length Taq polymerase. Takara Taq has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications.