Takara's DNA ligation Kit LONG is a powerful tool for cloning DNA fragments over 10 kb in length. The kit contains an optimized ligase/buffer system which enables ligation of long fragments without difficult techniques and special expertise. It is especially well-suited for the construction of BAC libraries.

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.

NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products can be prepared in a single reaction. This kit eliminates the need for pre-treatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.), of PCR products. This kit includes the same ligation reagents as are included in Takara's DNA Ligation Kit (Mighty Mix), which provide simple, quick, and efficient ligation. The kit is suitable for blunt-end cloning of PCR products obtained with PrimeSTAR HS and e2TAK DNA Polymerases (not available in all regions).