Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. This product is completely chemically defined and free of protein components. Because of this, the Western BLoT Blocking Buffer (Protein Free) provides higher sensitivity than other commonly used blocking agents, such as nonfat milk. In addition to detection of normal proteins, this blocking buffer is excellent for the detection of phosphorylated proteins. In addition, completely protein-free assays are possible when this product is used in combination with Western BLoT Immuno Booster PF (Cat. #T7115A). To obtain the highest detection sensitivity, combine with the Western BLoT HRP Substrate series.