Western BLoT Ultra Sensitive HRP Substrate allows detection of very small amounts of protein during Western blot analysis. This chemiluminescence substrate offers the highest sensitivity, and can be used to detect horseradish peroxidase (HRP)-labeled antibodies bound to target proteins on immunoblots. It is possible to detect protein levels in the femtogram range using this product. This substrate maintains a strong emission signal with linear signal across a wide dynamic range, allowing you to take full advantage of CCD camera detection sensitivity. Since background is very low, this system can be used for long exposure times, enabling detection of very small amounts of target protein. This product is useful when detecting minute amounts of target protein or when a limited amount of primary antibody is available. Western BLoT Ultra Sensitive HRP Substrate can be used for X-ray film detection. However, since the emission intensity is extremely high, it may be necessary to limit the exposure time and adjust the antibody dilution when using X-ray film.

Western BLoT Chemiluminescence HRP substrate is a horseradish peroxidase (HRP) substrate specially developed for X-ray film imaging of Western blots. This product is part of the Western BLoT HRP substrate series and produces a strong signal for high sensitivity with short exposure times. The signal from Western BLoT Chemiluminescence HRP substrate is long-lasting, allowing the blot to be re-imaged. For detection using a CCD imager or for applications requiring higher sensitivity, we recommend using other products in the Western BLoT HRP Substrate series.

The Western BLoT Quant HRP Substrate is a horseradish peroxidase (HRP) substrate specially developed for CCD imaging of Western blots. The signal from Western BLoT Quant is linear with respect to protein amount over a broad range of concentrations, allowing full use of the linear range of CCD detection. Western BLoT Quant produces a strong, extremely long-lived signal. These features, in combination with very low background levels, allow for long exposure times to enable the detection of low-abundance proteins. Western BLoT Quant is also compatible with X-ray film detection, although the limited dynamic range of film will make the resulting data less amenable to quantification. For applications requiring even higher sensitivity, we recommend using the Western BLoT Hyper HRP Substrate.

The Western BLoT Hyper HRP Substrate is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low-abundance proteins. Western BLoT Hyper produces a strong, long-lived signal. These features in combination with very low background levels, allows for long exposure times and enables the detection of low-abundance proteins. Additionally, the signal from this product is linear with respect to protein amount over a broad range of concentrations. Western BLoT Hyper HRP is more sensitive than Western BLoT Quant HRP Substrate, and is suitable for detection of low-abundance proteins or in situations when the amount of primary antibody is very limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. Western BLoT Hyper HRP is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less amenable to quantification.