Western BLoT Chemiluminescence HRP substrate is a horseradish peroxidase (HRP) substrate specially developed for X-ray film imaging of Western blots. This product is part of the Western BLoT HRP substrate series and produces a strong signal for high sensitivity with short exposure times. The signal from Western BLoT Chemiluminescence HRP substrate is long-lasting, allowing the blot to be re-imaged. For detection using a CCD imager or for applications requiring higher sensitivity, we recommend using other products in the Western BLoT HRP Substrate series.

The Western BLoT Hyper HRP Substrate is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low-abundance proteins. Western BLoT Hyper produces a strong, long-lived signal. These features in combination with very low background levels, allows for long exposure times and enables the detection of low-abundance proteins. Additionally, the signal from this product is linear with respect to protein amount over a broad range of concentrations. Western BLoT Hyper HRP is more sensitive than Western BLoT Quant HRP Substrate, and is suitable for detection of low-abundance proteins or in situations when the amount of primary antibody is very limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. Western BLoT Hyper HRP is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less amenable to quantification.