Production of sgRNA by in vitro transcription and testing for cleavage using recombinant Cas9 protein

sgRNA yield and quality for different gene targets using the Guide-it Complete sgRNA Screening System

sgRNA quality produced via in vitro transcription

Flow chart describing how the Guide-it sgRNA In Vitro Transcription Kit (including the Guide-it IVT RNA Clean-Up Kit) and Guide-it sgRNA Screening Kit work together in the Guide-it Complete sgRNA Screening System, which can be used to synthesize and test the efficacy of sgRNAs