SMART-Seq® Total RNA Mid Input
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis