The Library Quantification Kit is used for rapid library quantification prior to Illumina next-generation sequencing. The kit contains all of the components needed to determine the amount of library DNA (genomic, cDNA, etc.) in a sample using qPCR. Quantification is accomplished by comparison of the threshold cycle (Ct) of the diluted sample with a standard curve derived from four calibrated DNA standards.

The SMARTer-Seq Magnetic Separator - PCR Strip is designed especially for separation of magnetic beads (like AMPure XP beads; Beckman Coulter), from solution. This stand holds up to 24 0.2-ml PCR tubes in two rows, and is designed for capture of magnetic beads in 10–20 minutes. It is ideally suited for magnetic bead-based DNA purification and size selection techniques as used with most of our products for next-generation sequencing.

The SMARTer Human TCR a/b Profiling Kit enables users to analyze T-cell receptor (TCR) diversity from input RNA samples. This kit is designed to work with a range of RNA input amounts depending on the sample type, and has been shown to generate high-quality libraries from as little as 10 ng to 3 μg of total RNA obtained from peripheral blood leukocytes, or from 50 to 10,000 purified T cells. As the name suggests, the kit can be used to generate data for both alpha- and beta-chain diversity, either in the same experiment or separately.

This kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR transcripts. Included in the kit are primers that incorporate Illumina-specific adaptor sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms.