Representation of sgRNAs within the Guide-it CRISPR Genome-Wide sgRNA Library System.

Identification and analysis of sgRNAs isolated from cells after a 6-thioguanine screen.

Workflow schematic for an sgRNA library screen using 6-thioguanine (6-TG) selection.

Determination of transduction efficiency of the sgRNA library lentivirus using mCherry fluorescence.

Vector and sgRNA scaffold design used in the Guide-it CRISPR sgRNA library.