The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving procedures (NucleoSpin products) and processing of large sample volumes (NucleoSnap products).