NucleoVac 24 Vacuum Manifold
The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. Silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving protocols (NucleoSpin products) and the processing of large sample volumes (NucleoSnap products).
The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. Silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving protocols (NucleoSpin products) and the processing of large sample volumes (NucleoSnap products).
Setting up the NucleoVac 24
Figure 1. Setting up the NucleoVac 24 Vacuum Manifold. (1) Rubber closing plug. (2) Tubing connection. (3) Upper opening.
Figure 2. Processing columns with the NucleoVac 24 Vacuum Manifold. (4) NucleoSpin column. (5) Luer opening. (6) NucleoVac Mini Adapter. (7) NucleoVac Stopcock. (8) Luer plug.
NucleoVac 24 Vacuum Manifold procedure
Close the lower threaded opening of the NucleoVac 24 Vacuum Manifold (Figure 1) with the rubber closing plug (1). Screw in the tubing connection (2) in the upper opening (3) manually. Do not use a wrench as the conical screw thread might crack if the connection is over-tightened. Attach the vacuum tubing (not included).
For processing NucleoSpin (4) or NucleoSnap columns with the NucleoVac 24 Vacuum Manifold (Figure 2), insert NucleoSpin or NucleoSnap columns into the Luer openings (5; a). To avoid cross-contamination, we recommend using disposable NucleoVac Mini Adapters (6; b). For processing samples with different flow rates (e.g., large samle volumes), we recommend using NucleoVac Stopcocks (7; c) for maintaining constant vacuum pressure throughout the entire procedure. Close unused Luer openings with Luer plugs (8; d).
Overview
- Time-saving processing of 24 samples in parallel
- Parallel processing of silica columns (e.g., NucleoSpin and NucleoSnap columns) for nucleic acid purification
- Fast processing of large sample volumes
More Information
| Material | Polypropylene |
| Number of samples | 1–24 |
| Waste capacity | ≤900 ml |
| Column compatibility | NucleoSpin columns, NucleoSnap columns |
NucleoVac 24 and accessory product specifications
|
Product |
Size |
Specification |
Cat. # |
| NucleoVac 24 Vacuum Manifold | 1 each | NucleoVac 24 Vacuum Manifold, 24 NucleoVac Mini Adapters, 24 Luer plugs, 2 tubing connections, 1 closing plug | 740299 |
| NucleoVac Mini Adapters | 100 each | Luer adapters for connecting NucleoSpin or NucleoSnap columns to NucleoVac 24 Vacuum Manifold | 740297.100 |
| NucleoVac Stopcocks |
24 each |
Stopcocks for handling different flow rates of NucleoSpin and NucleoSnap columns on a NucleoVac 24 Vacuum Manifold | 740298.24 |
Applications
- Nucleic acid purification by vacuum
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes
Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.
Learn more Other plasmid kitsTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
