A fast way to purify plasmids for transfection—NucleoSpin Plasmid Transfection-grade

A fast way to purify plasmids for transfection that uses a new technology to reduce endotoxin levels.

NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses an innovative technology developed by Macherey-Nagel to reduce the level of endotoxins copurified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Click here to see performance data. A 96-well format is also available.

Cat. #	Product	Size	Price
740490.250	NucleoSpin® Plasmid Transfection-grade	250 Preps	USD $427.00
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Different plasmid purification technologies yield endotoxin levels appropriate for individual applications Different plasmid purification technologies yield endotoxin levels appropriate for individual applications. A quantitative chromogenic Limulus Amebocyte Lysate (LAL) test was used to assess endotoxin content. As indicated, endotoxin content depends strongly on the plasmid purification technology used. The NucleoSpin Plasmid Transfection-grade kit yields a plasmid solution containing sufficiently low endotoxin levels for direct transfection into common cell lines. . Back Compatibility of eluted DNA with transfection host cells Compatibility of eluted DNA with transfection host cells. A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH und Co. KG, Bielefeld, Germany) was purified from E. coli using the NucleoSpin Plasmid Transfection-grade kit. The purified plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or nucleofection (Lonza), with a >90% transfection ratio in both cases. Survival rates of cells transfected with the eluted DNA were compared to controls containing no added DNA. The similar ratios observed indicated that cell survival is not affected by addition of DNA eluates purified with the NucleoSpin Plasmid Transfection-grade kit. . Back The NucleoSpin Plasmid Transfection-grade procedure The NucleoSpin Plasmid Transfection-grade procedure. Back 740490.250: NucleoSpin Plasmid Transfection-grade
740490.50	NucleoSpin® Plasmid Transfection-grade	50 Preps	USD $102.00
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Different plasmid purification technologies yield endotoxin levels appropriate for individual applications Different plasmid purification technologies yield endotoxin levels appropriate for individual applications. A quantitative chromogenic Limulus Amebocyte Lysate (LAL) test was used to assess endotoxin content. As indicated, endotoxin content depends strongly on the plasmid purification technology used. The NucleoSpin Plasmid Transfection-grade kit yields a plasmid solution containing sufficiently low endotoxin levels for direct transfection into common cell lines. . Back Compatibility of eluted DNA with transfection host cells Compatibility of eluted DNA with transfection host cells. A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH und Co. KG, Bielefeld, Germany) was purified from E. coli using the NucleoSpin Plasmid Transfection-grade kit. The purified plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or nucleofection (Lonza), with a >90% transfection ratio in both cases. Survival rates of cells transfected with the eluted DNA were compared to controls containing no added DNA. The similar ratios observed indicated that cell survival is not affected by addition of DNA eluates purified with the NucleoSpin Plasmid Transfection-grade kit. . Back The NucleoSpin Plasmid Transfection-grade procedure The NucleoSpin Plasmid Transfection-grade procedure. Back 740490.50: NucleoSpin Plasmid Transfection-grade

Overview

Novel technology to diminish endotoxin content (<50 EU/µg DNA) for successful transfection
A miniprep purification format simplifies your workflow
Ultra-fast procedure

More Information

Technology	Silica membrane and endotoxin-reduction technologies
Format	Miniprep spin columns
Endotoxin content	<50 EU/µg DNA (EU=Endotoxin Units)
Starting material	Up to 5 ml bacterial culture (E. coli, high-copy plasmids)
Vector size	<15 kb
Typical yield	5–20 µg
A_260/A₂₈₀	1.80–1.85
Elution volume	30–50 µl
Preparation time	14 min/6 preps
Binding capacity	25 µg

Applications

Typical downstream applications: transfection of common cell lines (lipofection, electroporation)

Also suitable for standard molecular biology applications such as cloning and sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tip for a successful transfection—remove endotoxins!

Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.

View the data Purchase info

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A fast way to purify plasmids for transfection—NucleoSpin Plasmid Transfection-grade

Notice to purchaser

Different plasmid purification technologies yield endotoxin levels appropriate for individual applications

Compatibility of eluted DNA with transfection host cells

The NucleoSpin Plasmid Transfection-grade procedure

740490.250: NucleoSpin Plasmid Transfection-grade