New products from Takara Bio

Unlock full-length RNA isoforms from low-input samples

SMART-Seq mRNA Long Read

• Capture full-length transcripts up to 8 kb in length
• Process ~10 pg–100 ng of total RNA input or samples consisting of 1–1,000 cells
• Multiplex up to 96 samples for sequencing on the Oxford Nanopore Technology platform

Increase RNA purification throughput for plant and fungi samples

NucleoSpin 96 RNA Plant & Fungi

• Process samples in a 96-well format by centrifugation, vacuum, or automation
• Adapt the protocol with ease for your specific plant or fungal sample type
• Remove secondary metabolites that interfere with downstream processing

Achieve rapid and sensitive mycoplasma extraction

NucleoSpin Mycoplasma DNA

• Extract even trace amounts of mycoplasma DNA successfully from cell culture samples
• Have full trust in your results as every lot is confirmed to test negative for mycoplasma DNA
• Pair with the TaKaRa Mycoplasma qPCR Detection Kit for a complete mycoplasma solution

Discover fast, thermal-cycle-free amplification with high yields

phi29 DNA Polymerase

• Perform high-yield, isothermal amplification from as little as 5 pg of DNA
• Obtain accurate amplification from long DNA templates
• Skip optimization of PCR primer and cycling conditions

Get more from your NGS data with our latest bioinformatics software

Cogent NGS Analysis Pipeline / Cogent NGS Discovery Software

• Demultiplex scDNA-seq data in addition to single-cell and bulk RNA-seq data
• Perform ribodepletion of data using SortMeRNA
• Analyze data generated from the high-throughput Shasta Total RNA-Seq and the Shasta Whole-Genome Amplification kits

Ultra-long amplification PCR enzyme with high fidelity and specificity

PrimeSTAR LongSeq DNA Polymerase

• Amplify ultra-long (50 kb or more) segments—even difficult sequences such as GC-/AT-rich sequences
• Suppress nonspecific amplification—for highly specific long-length multiplex PCR reactions
• Leverage highly accurate template amplification—ideal for long-read sequencing

Simplify automation workflows for clinical and veterinary samples

NucleoMag Pathogen/VET prefilled plates for IsoPure Mini

• Process 8–32 samples in parallel
• Minimize hands-on time and handling mistakes
• Reduce plastic consumption by up to 50%

Achieve fast and highly accurate RT-PCR in a single tube

PrimeScript II High Fidelity One Step RT-PCR Kit

• Perform a high-speed reaction with reverse transcription in 10 min and a PCR extension time of 10 sec/kb
• Amplify GC-rich and long cDNA with high accuracy
• Use with a wide range of total RNA amounts

High-throughput biomarker discovery with Shasta technologies

Shasta Single Cell System

• Prevalidated applications for high-throughput single-cell whole-genome or transcriptome profiling
• Intuitive user interface for setting up custom NGS assays with just a few clicks
• Large-bore nozzle for unbiased cell isolation, even for challenging cell types such as primary cardiomyocytes
• End-to-end solution with accompanying bioinformatics analytical tools

Scale up mRNA production with low dsRNA byproducts

PrimeCap T7 RNA Polymerase (low dsRNA), HQ

• Obtain in-vitro transcribed RNA that is notably safer by reducing dsRNA byproducts by >90%
• Retain the same high yields as T7 RNA polymerase ver.2, especially when used with cap analogs such as CleanCap Reagent AG (TriLink Bio Technologies)
• Facilitate a smooth transition to large-scale manufacturing with enzymes produced following many key features of GMP guidelines