Unlock immunological insights to reveal disease mechanisms

In this workshop, Dr. Yue Yun will discuss how our SMART (Switching Mechanism at 5' end of RNA Template)-based immune profiling technologies deliver the sensitivity and accuracy needed to discover TCR and BCR biomarkers. Learn how our solutions achieved superior detection of rare BCR clonotypes in a rigorous spike-in study and the real-world applications of SMART tools to track immune dynamics during disease. Join us to discover how to transform your research with advanced immune profiling tools.

This poster shows how Trekker™ was used to transform single-cell sequencing assays into spatially resolved single-cell data. This technology utilizes unique DNA barcodes on a tightly packed bead array to spatially label individual cells. The resulting tagged nuclei are then processed on conventional single-cell sequencing platforms, preserving spatial information while providing true single-cell resolution. In this study, Trekker was applied to achieve spatial ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) combined with gene expression analysis, as well as spatial V(D)J sequencing paired with gene expression.

This poster shows that SMART-Seq Human BCR (with UMIs) effectively detected specific recombination events for heavy chain isotypes across nine monoclonal B-cell lines, at frequencies corresponding to spike-in percentages or known expression levels. Deep analysis of BCR repertoires can provide profound insight into both healthy and diseased immune systems.