His-tagged protein purification
Over 75% of researchers use his tags for purifying proteins due to their many advantages, including their small size (making them less likely to alter wild-type protein function/structure and easy to clone), their effectiveness when incorporated on either N- or C-termini, and their suitability for purification of both native and denatured proteins.
Protein purification technologies
TALON resin
Choose the right TALON resin format.
His60 Ni resin
Choose the right His60 Ni resin format.
Technical notes
Purification of active, secreted proteins
Purify active, secreted his-tagged proteins from cell-culture supernatants in 15 minutes.
A revolutionary solution for protein purification
Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.
Capturem large volume
A simple, rapid method for purifying and concentrating his-tagged protein from large sample volumes.
Magnetic beads for protein purification
Magnetic beads enable one-step protein purification and pull-down assays.
Obtain highest purity with cobalt resin
Prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells.
His-tag learning center
Different tags: His, GST, and Myc
Explore the benefits of using peptide tags and options for tagged protein purification.
Comparison of purification methods
Learn how to choose the best his-tagged protein purification method for your specific application.
TALON resin FAQs
FAQs about TALON resin, which yields more highly purified his-tagged proteins than traditional Ni-NTA resins.
Why use His60?
Discover how to improve protein purity from Ni resin purification
Protocols
Learn about our quick and easy protein purification kit protocols.
Purification tips and tricks
How to choose the right his-tagged purification resin
Learn how to decide between nickel- and cobalt-based IMAC resins for your his-tagged purification.
When your his-tagged constructs don’t bind—troubleshooting your protein purification woes
This blog post covers reasons his-tag fusions might not bind to resin and how to tackle these issues.
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