Protein expression overview

E. coli systems:
Up to 1 g/l

High-yield bacterial systems: 
Up to 25 g/l

• Get simple, rapid, high-yield protein expression.

• Choose from E. coli systems that incorporate his or HAT tags to enable efficient IMAC purification, and that provide inducible expression, better solubility, fast cloning, included purification resins and reagents, high-binding capacity, or high purity.

• Choose from a variety of bacterial systems that offer high yields, high purity, fast cloning, or the ability to express proteins that are insoluble, toxic, or contain disulfide bonds.

Standard system: 
Up to 50 µg/20 µl

Maxi* system: 
Up to 900 µg/80 µl

*Product availability varies by region.

• Obtain fast, easy, single-tube protein expression in as little as 1 hour with a human cell-free system that expresses proteins with the appropriate post-translational modifications needed for biological activity.
• Express large proteins (>150 kDa), as well as proteins that are degraded, insoluble, or toxic using in vivo systems.

• Achieve high yields of recombinant protein, with post-translational processing, biological activities, and immunological reactivities similar to mammalian systems.
• Express proteins that are difficult to express in bacteria due to size, complexity, or post-translational modifications.

• Enable efficient immunoprecipitation and affinity purification by incorporating Myc-, or HA-fusion tags.
• Express proteins that fold and assemble correctly, and contain the appropriate post-translational modifications needed for biological activity.