Learning centers
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Automation systems
- Shasta Single Cell System introduction
- SmartChip Real-Time PCR System introduction
- New and improved high-throughput qPCR technology
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ICELL8 introduction
- ICELL8 cx applications
- ICELL8 technology overview
- ICELL8 cx technical specifications
- ICELL8 technical specifications (original system)
- ICELL8 system vs plate-seq
- Webinars
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Technical notes
- Enhancing biomarker discovery with SMART-Seq Pro kit and ICELL8 cx system
- ICELL8 cx system target enrichment for fusions
- ICELL8 cx system reagent formulation and dispense guidelines
- Improved detection of gene fusions, SNPs, and alternative splicing
- Full-length transcriptome analysis
- High-throughput single-cell ATAC-seq
- Protocol: High-throughput single-cell ATAC-Seq
- Single-cell identification with CellSelect Software
- Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
- Combined TCR profiling and 5’ DE in single cells
- Automated, high-throughput TCR profiling
- Sample preparation protocols
- Video resources
- Citations
- Posters
- System & software notices
- Apollo library prep system introduction
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Next-generation sequencing
- Product line overview
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Technical notes
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DNA-seq
- High-resolution CNV detection using PicoPLEX Gold DNA-Seq
- Next-gen WGA method for CNV and SNV detection from single cells
- Low-volume DNA shearing for ThruPLEX library prep
- Low-input whole-exome sequencing
- Cell-free nucleic acid sequencing
- DNA-seq from FFPE samples
- ThruPLEX FLEX data sheet
- Improvements to ThruPLEX HV
- ThruPLEX FLEX outperforms NEBNext Ultra II
- Comparing ThruPLEX HV PLUS to Kapa and NEBNext
- Low cell number ChIP-seq using ThruPLEX DNA-Seq
- Detection of low-frequency variants using ThruPLEX Tag-Seq FLEX
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Immune Profiling
- Track B-cell changes in your mouse model
- Efficient and sensitive profiling of human B-cell receptor repertoire
- TCRv2 kit validated for rhesus macaque samples
- Improved TCR repertoire profiling from mouse samples (bulk)
- TCR repertoire profiling from mouse samples (bulk)
- BCR repertoire profiling from mouse samples (bulk)
- Improved TCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from human samples (single cells)
- BCR repertoire profiling from human samples (bulk)
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RNA-seq
- Singular for low input total RNA seq
- All-in-one cDNA synthesis and library prep from single cells
- Automation-friendly, all-in-one cDNA synthesis and library prep
- All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
- 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
- Full-length mRNA-seq for target capture
- Stranded libraries from single cells
- Stranded libraries from picogram-input total RNA (v3)
- Stranded libraries from 100 pg-100 ng total RNA
- Stranded libraries from 100 ng - 1 ug total RNA
- Stranded libraries from FFPE inputs (v2)
- Nonstranded libraries from FFPE inputs
- Singular and Takara Bio library prep
- Full-length, single-cell, and ultra-low-input RNA-seq with UMIs
- Epigenetic sequencing
- Reproductive health technologies
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DNA-seq
- Technology and application overviews
- FAQs and tips
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DNA-seq protocols
- Using UMIs with ThruPLEX Tag-Seq FLEX
- Exome capture with Illumina Nextera Rapid Capture
- Targeted capture with Roche NimbleGen SeqCap EZ
- Targeted capture with IDT xGen panels
- Targeted capture with Agilent SureSelectXT
- Targeted capture with Agilent SureSelectXT2
- Targeted capture with Agilent SureSelectQXT
- Plasma preparation for ThruPLEX Plasma-Seq workflow
- Bioinformatics resources
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Webinars
- Harnessing the power of full-length transcriptome analysis for biomarker discoveries
- SMART-Seq Pro kits for biomarker detection
- Takara Bio Single-Cell Workshop, Spring 2021
- Capturing biological complexity by high-resolution single-cell genomics
- Sensitivity and scale for neuron multiomics
- Taking single-cell RNA-seq by STORM
- Pushing the limits of sensitivity for single-cell applications
- Liver metabolic function, dissecting one cell at a time
- Single-Cell Workshop at 2020 NextGen Omics Series UK
- Immunogenomics to accelerate immunotherapy
- Total RNA sequencing of liquid biopsies
- MeD-Seq, a novel method to detect DNA methylation
- Automating full-length single-cell RNA-seq libraries
- Single-cell DNA-seq
- Single-cell whole transcriptome analysis
- Posters
- mRNA and cDNA synthesis
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Gene function
- Gene editing
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Viral transduction
- Recombinant virus comparison
- Product finder
- Transduction posters
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Lentivirus
- Lenti-X Transduction Sponge
- Webinars
- Customizable SARS-CoV-2 pseudovirus
- Lenti-X FAQs
- Lentiviral workflow
- Lentiviral products guide
- Lentivirus biosafety
- Lentiviral tips
- Lentiviral vectors
- Lenti-X packaging
- High-throughput lentivirus production
- Lenti-X Concentrator
- Lentiviral titration
- Lenti-X GoStix Plus video protocols
- Lenti-X GoStix Plus FAQs
- Rapid lentivirus titration by p24 ELISA
- Lentiviral particles
- Lentiviral particles—fluorescent
- Lentiviral particles FAQs
- Retrovirus
- Adeno-associated virus
- Adenovirus
- T-cell transduction and culture
- Inducible systems
- Transfection reagents
- Fluorescent proteins
- Cell biology assays
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Stem cell research
- Overview
- Protocols
- Applications
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Technical notes
- Pluripotent stem cells
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Gene editing in hiPS cells
- Tagging an endogenous gene with AcGFP1 in hiPS cells
- Tagging an endogenous gene with a myc tag in hiPS cells
- Generating clonal hiPS cell lines deficient in CD81
- Introducing a tyrosinemia-related SNP in hiPS cells
- Inserting an expression cassette into the AAVS1 locus in hiPS cells
- Editing hiPS cells using electroporation
- Editing hiPS cells using gesicle technology
- Single-cell cloning of hiPS cells
- Organoids
- Beta cells
- Hepatocytes
- Cardiomyocytes
- Neural stem cells
- Posters
- Webinars
- Videos
- FAQs
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Citations
- Cellartis MSC Xeno-Free Culture Medium
- Cellartis Power Primary HEP Medium
- Cellartis DEF-CS 500 Culture System
- Cellartis Enhanced hiPS-HEP cells
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- GS1-R
- 2i mES/iPSC medium
- iMatrix-511
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- RHB-A
- STEM101
- STEM121
- STEM123
- Selection guides
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Protein research
- Capturem technology
- Antibody immunoprecipitation
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His-tag purification
- Purification methods overview
- TALON resin selection guide
- Selection guide: His60 resin
- xTractor Buffer is optimized for superior protein yield
- Why tag a protein?
- Tech note: cobalt resin
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- FAQs: TALON
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Protocols
- Video: Capturem his maxiprep
- Video: Capturem his miniprep
- Visual protocol: Capturem his maxiprep
- Visual protocol: Capturem his miniprep
- Capturem nickel column reagent compatibility
- TALON reagent compatibility
- His60 reagent compatibility
- TALON: Native vs denaturing purification
- Protocol: denaturing purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, pH elution
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
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PCR
- Citations
- PCR selection guide
- PCR enzyme brochure
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Technical notes
- Rapid, high-performance multiplex PCR
- EmeraldAmp outperforms MyTaq Red mix
- Fast and accurate PCR
- Methylation studies
- Hot-start PCR
- Long-range PCR with LA Taq
- Direct PCR from human nail
- Direct PCR from meat samples
- Megaprimer PCR with PrimeSTAR GXL
- Amplifying GC-rich templates
- Titanium Taq for high-throughput genotyping
- Colony PCR in under an hour
- High-throughput endpoint PCR
- Direct PCR from blood
- PrimeSTAR GXL for targeted sequencing
- Detecting somatic mosaicism using massively parallel sequencing
- FAQ
- Go green with lyophilized enzymes
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Cloning
- Automated In-Fusion Cloning
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In-Fusion Cloning general information
- In-Fusion Cloning overview
- In-Fusion Cloning guide
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In-Fusion Cloning and competition
- In-Fusion Snap Assembly vs. GeneArt Gibson Assembly HiFi
- In-Fusion Snap Assembly vs. NEBuilder HiFi
- Sequence accuracy in seamless cloning
- Choosing a seamless cloning method
- Improving background over Gibson Assembly
- A successful alternative to ligation cloning
- Single- and multiple-insert cloning
- Easy cloning into lentiviral vectors
- Outperforming TOPO cloning
- In-Fusion Cloning citations
- Stellar Competent Cells product overview and performance data
- EcoDry reagents and sustainability
- Primer design and other tools
- In‑Fusion Cloning tips and FAQs
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Applications and technical notes
- In-Fusion Cloning applications collection
- Efficient multiple-fragment cloning
- Mutagenesis with In-Fusion Cloning
- Rapid, high-throughput cloning for antibody development
- Solve a synthesis challenge with easy multiple-insert cloning
- Direct cloning into large vectors
- Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
- Efficient cloning for sgRNA/Cas9 plasmids
- In-Fusion Cloning of sgRNAs
- De novo insertion of small fusion protein tags
- Sign up to stay updated
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Traditional molecular cloning
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Restriction enzyme overview
- General information about restriction enzymes
- Star activity of restriction enzymes
- Inactivation of restriction enzymes
- Buffer activity with restriction enzymes
- Universal buffers for double digestion with restriction enzymes
- Restriction enzymes affected by methylation
- Methylation-sensitive restriction enzymes
- QC of restriction enzymes
- Ligation cloning overview
- Ligation product guide
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Restriction enzyme overview
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Nucleic acid purification
- Nucleic acid extraction webinars
- Product demonstration videos
- Plasmid kit selection guide
- Plasmid purification
- Genomic DNA purification
- DNA/RNA cleanup and extraction
- RNA purification
- RNA purification kit finder
- Viral DNA and RNA purification
- Parallel DNA, RNA & protein
- Automated DNA and RNA purification
- Accessory selection guides
- Microbiome
- Antibodies and ELISA
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Real-time PCR
- Overview
- Product finder
- Reaction size guidelines
- Real-time PCR products brochure
- Real-time PCR tutorial videos
- Guest webinar: extraction-free SARS-CoV-2 detection
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Technical notes
- Unbiased preamplification of limited samples
- Accurate gene expression analysis with TB Green Premix Ex Taq II
- Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
- Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
- Monitoring siRNA knockdown
- qPCR without optimization using TB Green Premix Ex Taq
- Fast synthesis of cDNA templates for real-time RT-PCR
- Specific, consistent real-time PCR with TB Green Premix Ex Taq II
- Mir-X microRNA quantification
- FAQs
Real-time PCR learning center
A long history of innovative research supports our real-time qPCR reagents. Explore below to learn about qPCR, find out how our technology works, view real-world technical data generated with our kits, and browse selection guides to find the right tool for your research.
Technical notes
- Unbiased preamplification of limited samples
- Accurate gene expression analysis with TB Green Premix Ex Taq II
- Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
- Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
- Monitoring siRNA knockdown
Unlock answers with nanoscale PCR
Takara Bio's SmartChip ND Real-Time PCR System allows you to flexibly design your own panels, keep costs low, and obtain results in under 3 hr. Sample dispensing and reaction mix distribution is automated for up to 5,184 reactions per chip. Customizable configurations allow 12 to 384 assays to be processed at a time, depending on the number of targets in the panel. The system also simplifies your workflow with full end-to-end software to carry out automated dispensing and qPCR analysis.
Learn moreTakara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
