Restriction enzyme overview
With 30 years of production experience, our DNA restriction enzymes are unsurpassed in quality and purity. Every lot of our DNA restriction enzymes undergoes stringent quality control tests to ensure that the enzymes are supplied with optimized buffers for maximum activity during digestion reactions.
General information about restriction enzymes
Instructions for the use, overview of common issues, and descriptions of restriction enzyme QC tests.
Compensating for star activity of restriction enzymes
Use the table on this page to find ways to avoid star activity.
Inactivation and residual activity of restriction enzymes
Residual enzyme activity following inactivation treatment
Effects of methylation on the activities of restriction enzymes
Characteristics of restriction enzymes affected by Dam methylase, Dcm methylase or CG methylase-mediated methylation.
Relative activity of restriction enzymes in universal and basal buffers
Table of relative activities of restriction enzymes in universal and basal buffers; compositions and instructions for use of buffers.
Universal buffers for double digestion with restriction enzymes
Recommended universal buffers for double digestion with restriction enzymes
Methylation-sensitive restriction enzymes (MSREs)
Methylation-sensitive restriction enzymes (MSREs) for use in mammalian epigenetics analyses with CpG methylase
Restriction enzyme quality control
We perform four types of tests to assure the quality of our restriction enzymes
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