GC-rich PCR products

GC-rich templates, such as the 5' ends of many mammalian cDNAs, are difficult to amplify using conventional PCR techniques due the presence of secondary structures that resist denaturation and prevent efficient primer annealing. Takara Bio offers several DNA polymerases designed to handle such challenging targets, with amplification of templates that contain up to 90% GC content.

Featured product

PrimeSTAR GXL DNA Polymerase

Perform PCR with superior accuracy, sensitivity, and specificity—even with GC-rich templates. Easily amplify products ≤30 kb.

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Product pages

PrimeSTAR GXL DNA Polymerase

PrimeSTAR HS with GC buffer

LA Taq DNA polymerase with GC buffers

Advantage GC 2 polymerase

Advantage GC Genomic LA Polymerase Mix

How the leopard lost its spots

Why do the offspring of some leopards hide their spots, resulting in black panthers? An international team of researchers focused on ASIP, a gene that causes dark coat coloration in other felines. Takara Bio's LA Taq with GC Buffer was used to sequence a tricky stretch of exon 4 in leopards and Asian golden cats, identifying SNPs associated with melanistic coat color. Scientists can now answer the curious question of how leopards produce panthers. That's Good Science!

Featured product: Takara LA Taq DNA Polymerase paired with GC-optimized buffers

Tech notes

Superior amplification of GC-rich templates with PrimeSTAR GXL DNA Polymerase

View data on the amplification of GC-rich templates without reaction optimization or inclusion of additives such as DMSO or Betaine.

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Tech note

Amplification and cloning of a low-expression antibody heavy-chain variable region gene (IGHV4-59) using megaprimer-mediated domain swap

View data on using PrimeSTAR GXL for megaprimer PCR.

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