SpeedSTAR HS DNA Polymerase
SpeedSTAR HS DNA Polymerase is optimized for fast PCR and does not require specialized PCR equipment. With an extension rate as fast as 10 sec/kb (compared to 60 sec/kb with standard Taq DNA polymerase), SpeedSTAR DNA polymerase dramatically reduces total reaction time and can be used with standard PCR machines.
SpeedSTAR HS DNA Polymerase includes two optimized buffers, Fast Buffer I and II, to provide efficient amplification across a wide size range (up to 20 kb) with less optimization than other polymerases. The antibody-mediated hot-start formulation provides high specificity. A dNTP mixture also is supplied with the polymerase.
Overview
- Optimized for fast PCR: amplify a 2-kb fragment in as little as 30 minutes using a standard PCR machine
- Excellent efficiency: robust performance, comparable to high-yield polymerases
- Optimized buffers allow amplification of fragments up to 20 kb
More Information
Applications
- Fast PCR: reactions can be completed in two-thirds less time
- Amplification of large DNA fragments
PCR products
The majority of PCR products generated with SpeedSTAR DNA Polymerase will have 3'-A overhangs; therefore, amplification products can be directly cloned into T-vectors. It is also possible to clone amplification products into blunt-ended vectors following blunting and phosphorylation of product ends.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Speeding up forensic analysis—That's Good Science!
Researchers for the Royal Canadian Mounted Police looked to high-speed polymerases as a potential tool for sample analysis. SpeedSTAR HS polymerase brought their PCR processing time from four hours down to 26 minutes!
Watch the video
See what our customers are saying about SpeedSTAR HS DNA Polymerase!
"It worked with a fast protocol I have used for a new design of a thermal cycler. My goal is to make PCR as fast as possible to cut down the overall time of a reactions. I wonder if the SpeedSTAR will work well with real-time reaction in the presence of dyes."
—Season Wong, AI BIOSCIENCES INC
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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