PrimeSTAR GXL Premix
PrimeSTAR GXL Premix is a convenient 2X PCR master mix of Takara's PrimeSTAR GXL DNA polymerase. PrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.
PrimeSTAR GXL Premix is a convenient 2X PCR master mix of Takara's PrimeSTAR GXL DNA polymerase. PrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.
PrimeSTAR GXL Premix enables you to simplify your amplification workflow, saving you time and reducing potential errors. Simply add primers and template, and you are ready to go.
Overview
- Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
- Antibody-mediated hot-start formulation
- High tolerance for GC-rich templates as well as the presence of excess nucleic acid templates
More Information
Applications
- Realiable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
- HLA typing
- Unbiased library amplification for NGS
Termini of PCR products
- PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.
Recommended maximum amplicon length
- Up to 30 kb (human genomic DNA template)
- Up to 40 kb (lambda DNA template)
- Up to 13.5 kb (human cDNA template)
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Powered by BiozTech note
Long PCR for cost-effective and efficient targeted next-gen sequencing.
Long-range PCR with PrimeStar GXL DNA Polymerase was used to amplify the entire BRCA1 and BRCA2 genes for targeted next-gen sequencing.
Tech note
Amplification and cloning of a low-expression antibody heavy-chain variable region gene (IGHV4-59) using megaprimer-mediated domain swap
View data on using PrimeSTAR GXL for megaprimer PCR.
See what our customers are saying about PrimeSTAR GXL DNA Polymerase!
This enzyme allowed me to amplify the full-length of some 4+kb cDNA that was not successfully amplified with the other company's conventional Taq DNA polymerase. Both specificity and capability to amplify long sequences seem to be superior. We already ordered this product. Thank you for providing the sample prior to make a decision."
—Katsuhiro Kita, WEILL CORNELL MEDICAL COLLEGE
This is a good product that is reasonably priced. I am hoping to have grant fundong by the fall of 2013, and by then I plan to use this products in my research."
—Farid Ahmed, GEM TOX LABS
We are using Expand Long Template kit for our fusion PCRs. we have tried many other enzymes but have not had any success. PrimeSTAR® GXL DNA Polymerase performed better than our existing polymerase."
—Aysha Osmani, OHIO STATE UNIVERSITY
Find high-quality solutions for applications downstream of your PCR
Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.
Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.
Clean itFind answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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