Premix Ex Taq
Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of Takara Ex Taq polymerase blend, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.
Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of Takara Ex Taq polymerase blend, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.
A loading dye-added 2X master mix (PerfectShot Ex Taq DNA Polymerase) is available for convenient loading of PCR products in agarose gels.
Ex Taq is also available in the following formats:
- Individual components—including Ex Taq polymerase mix, dNTP mixture, and 10X reaction buffer (with or without Mg2+) for individual reaction setup and optimization.
- An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
Overview
- High-yield, high-sensitivity PCR
- Premixed PCR master mix for convenient amplification requiring minimal assembly
- Generation of PCR products having >80% cloning efficiency in T-vectors
More Information
Applications
- Ideal for high-fidelity, high-yield endpoint PCR
PCR products
PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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