TaKaRa Z-Taq DNA Polymerase

TaKaRa Z-Taq DNA Polymerase enables rapid PCR amplification due to its greater processivity relative to standard Taq DNA polymerase. Z-Taq polymerase can amplify a 1-kb DNA fragment from genomic DNA in 20 minutes, using a 30-cycle, two-step amplification protocol. The improved processivity of the Z-Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase (up to 20 kb from lambda DNA). This kit includes separate tubes of enzyme, buffer, and dNTPs.

Cat. #	Product	Size	Price
R006B	TaKaRa Z-Taq™ DNA Polymerase	800 Units	USD $1360.00
Takara Z-Taq is supplied with separate tubs of optimzed buffer (Mg²⁺ plus), and dNTPs. Takara Z-Taq is characterized by 5-fold greater processivity than Taq DNA Polymerase, resulting in rapid amplification times in PCR applications. Cat. # R006B contains 4 of Cat. # R006A. Please refer to Cat. # R006A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
R006A	TaKaRa Z-Taq™ DNA Polymerase	200 Units	USD $389.00
Takara Z-Taq is characterized by 5-fold greater processivity than Taq DNA Polymerase, resulting in rapid amplification times in PCR applications. Separate tubs of optimzed buffer (Mg²⁺ plus), and dNTP mix are supplied with the polymerase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back A 1 kb fragment of the G3PDH gene was amplified from 0 A 1 kb fragment of the G3PDH gene was amplified from 0.1, 1, 10, or 100 ng of human placenta DNA. The cycling conditions were 30cycles of 98° C, 5 seconds; 68°C, 20 seconds. After PCR, 5 µl of each reaction mixture was loaded on an agarose gel for electrophoresis. The results indicate that Z-Taq DNA Polymerase could amplify the 1 kb target from as little as 1 ng of template DNA. Back Amplification of long products using Takara Z-Taq DNA Polymerase Amplification of long products using Takara Z-Taq DNA Polymerase. Long products (7.5 kb, 17.5 kb, 18 kb, or 20 kb) were amplified from human genomic DNA (500 ng/50 µl reaction) or E. coli genomic DNA (100 ng/50 µl reaction) using the following conditions: [Human 7.5 kb product] 30 cycles of: 98°C, 5 sec.; 68°C, 80 sec. *[E. coli* 18 and 20 kb products and human 17.5 kb product]** 35 cycles of: 98°C, 5 sec.; 68°C, 180 sec. All reactions were performed using a TaKaRa PCR Thermal Cycler PERSONAL (discontinued) using the FAST cycling mode. After PCR, 5 µl of the reaction mixture was loaded on an agarose gel for electrophoresis. Takara Bio's Z-Taq DNA Polymerase efficiently amplified large genomic fragments, including a 20 kb target. Back R006A: TaKaRa Z-Taq DNA Polymerase

Overview

Reaction speed five times faster than standard Taq DNA polymerase

Amplify human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb

More Information

Applications

Fast PCR

PCR products

PCR products generated with Takara Z-Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency when using T-vectors.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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TaKaRa Z-Taq DNA Polymerase

Notice to purchaser