TaqStart Antibody: turn any Taq into a hot-start Taq
TaqStart Antibody provides antibody-mediated hot start that enhances the specificity and sensitivity of PCR. This antibody inhibits polymerase activity before the onset of thermal cycling, preventing nonspecific amplification and primer-dimer formation. When the reaction temperature is raised, the antibody is quickly inactivated and PCR proceeds. Dilution buffer is provided.
TaqStart Antibody provides antibody-mediated hot start that enhances the specificity and sensitivity of PCR. This antibody inhibits polymerase activity before the onset of thermal cycling, preventing nonspecific amplification and primer-dimer formation. When the reaction temperature is raised, the antibody is quickly inactivated and PCR proceeds. Dilution buffer is provided.
TaqStart Antibody is significantly more convenient to use than other hot-start methods and offers several advantages:
- Avoids sample damage due to depurination that can occur with the high-temperature incubations that are necessary to activate some hot-start enzymes (Fromenty et al. 2000; Barnes 1994; Fromenty et al. 1996; Friedberg, Walker, and Siede 1995; Lindahl and Andersson 1972; Suzuki, Ohsumi, and Makino 1994).
- Reduces the risk of cross-contamination because it is not necessary to reopen the reaction tubes after heating.
- Can be used when other hot-start methods are difficult to perform, such as for high-throughput PCR, in situ PCR, microtiter-plate formats, capillary PCR, and oil or wax-free environments.
- Provides more definitive PCR results in cases when amplification of nonspecific products is a problem, such as reactions involving low copy number targets, complex DNA background, or degenerate primers.
TaqStart Antibody is effective with any Taq-derived DNA polymerase (native, recombinant, and N-terminal deletion mutants). Our Titanium Taq DNA Polymerase and all of our Advantage HD and Advantage 2 products include TaqStart Antibody.
Overview
- Allows convenient room temperature reaction setup
- Provides increased specificity, which leads to reduced background, allowing you to obtain your desired amplification product the first time
- Effective and inexpensive method for hot-start PCR
- Antibody can be used with any full-length Taq polymerase
- Available in bulk quantities
More Information
Applications
- Hot-start PCR
References
Barnes, W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. U. S. A. 91, 2216–20 (1994).
Friedberg, E. C., Walker, G. C. & Siede, W. DNA repair and mutagenesis (ASM Press, 1995).
Fromenty, B. et al. Most cases of medium-chain acyl-CoA dehydrogenase deficiency escape detection in France. Hum. Genet. 97, 367–8 (1996).
Fromenty, B., Demeilliers, C., Mansouri, A. & Pessayre, D. Escherichia coli exonuclease III enhances long PCR amplification of damaged DNA templates. Nucleic Acids Res. 28, E50 (2000).
Lindahl, T. & Andersson, A. Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid. Biochemistry 11, 3618–23 (1972).
Suzuki, T., Ohsumi, S. & Makino, K. Mechanistic studies on depurination and apurinic site chain breakage in oligodeoxyribonucleotides. Nucleic Acids Res. 22, 4997–5003 (1994).
Product citations
Gerard, G. F., D'Alessio, J. M. in Methods Mol. Biol. (Burrell, M. M.) 73–94 (Humana Press, 1993).
Kellogg, D. E. et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16, 1134–7 (1994).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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