ICELL8 Single-Cell System technical specifications

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Automation systems

Technical specifications

The original ICELL8 Single-Cell System is an automated, open platform for high-throughput single-cell isolation, visualization, and processing. The system consists of two major hardware components: the ICELL8 MultiSample NanoDispenser (MSND) and the ICELL8 Imaging System. Cell and reagent dispensing into 5,184-nanowell chips is automated using the ICELL8 MSND, which can accommodate up to eight samples at a time. The ICELL8 Imaging System is designed for scanning and acquiring images of samples (e.g., cells, nuclei, or spheroids) dispensed into an ICELL8 chip. Use of the system's imaging channels with cellular dyes allows for assessment of cell viability.

ICELL8 MSND specifications ICELL8 Imaging System specifications

ICELL8 MSND specifications

Category	Specifications
Dispense volume	35–50 nl or 100 nl per well
Laptop computer	Windows 7 2 GB memory 120 GB storage 1 GB network adapter USB ports for memory sticks
Power requirements	One of the following options: 120 VAC/60 Hz mains: one 120V, 15 or 20A circuit; three NEMA 5-15 receptacles are required for the fluidic module, pump box, and laptop computer (the humidifier plugs into the pump box, and thus a separate receptacle is not required) 220–240 VAC/50 Hz mains: one 10A circuit to power a 230:115 step-down transformer and NEMA 5-15 power strip (transformer and power strip are supplied with the system); transformer adapters will be supplied for continental Europe (Schuko type), UK, and China installations 100 VAC/50–60 Hz mains: one 15A circuit to power a 100:120 step-up transformer and NEMA 5-15 power strip (transformer and power strip are supplied with the system)
Dispenser fuses	5 x 20 mm, T5H 1.6 watts/6.3A max 250V
Environmental conditions	15–30°C ambient 30–70% relative humidity, noncondensing <2,000 m above sea level <2 pollution degree
Dimensions	Laptop computer: (W) 34 cm, (H) 5 cm, (D) 25 cm Fluidic module: (W) 28 cm, (H) 33 cm, (D) 45 cm Stage module: (W) 27 cm, (H) 40 cm, (D) 60 cm Peristaltic pump control box: (W) 26 cm, (H) 38 cm, (D) 51 cm Humidifier: (W) 41 cm, (H) 81 cm, (D) 66 cm Helium source: 25-cm diameter cylinder; (H) 152 cm Waste container: (W) 22 cm, (H) 36 cm, (D) 15 cm
Weight	65 kg

ICELL8 Imaging System specifications

Category	Specifications
Power requirements	100–240 VAC, 2.0/1.0 A (47–63 Hz), power outlet within 2 m Main supply voltage with ≤10% variance from nominal
Dispenser fuses	5 x 20 mm, T5H 1.6 watts/6.3A max 250V
Environmental conditions	15–30°C ambient 10–70% relative humidity, noncondensing <2 pollution degree
Dimensions	(W) 163 cm, (H) 86 cm, (D) 66 cm

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