Demo video: NucleoBond PC 10000 and NucleoBond PC 10000 Rack
Learn how the NucleoBond PC 10000 kits can help in large-scale purification of up to 10 mg of ultrapure, endotoxin-free plasmid DNA.
Simplify plasmid purification steps in your vaccine, gene therapy & AAV production workflows
Product overview | Endotoxins | Applications | Demo video
NucleoBond PC 10000 EF overview
Lysates are cleared by simple filtration using the included NucleoBond Folded Filters—no centrifugation is required.
Endotoxins
Endotoxins (lipopolysaccharides [LPS]) are heat-stable toxins found in the outer membranes of gram-negative bacteria. LPS trigger a strong reaction in mammalian immune systems that can contribute to low-level inflammatory-related diseases or life-threatening states such as septic shock.
| Endotoxins can lead to the release of cytokines, such as: | Induced changes due to endotoxins: | |||
|
Cell type | Endotoxin unit (EU) |
Effect | |
|
Urethral epithelium |
25,000 |
Alters clonal efficiency | |
|
Human T cell | 500 | Induces proliferation and lymphokine production | |
|
Murine B lymphoma | 50 | Increases production of light-chain immunoglobulin 30–40 fold | |
|
Recombinant CHO | 50 | Alters production of protein | |
|
Human IVF embryo | 5 | Reduces success rates of in vitro fertilization programs 3–4 fold | |
|
Aortic ring | 5 | Reduces cardiac contractility, induces production of IL-1 and tumor necrosis factor | |
|
Equine macrophage | 2.5 | Induces production of IL-6 | |
Applications
Vaccine production workflow, from viral sequencing to in vitro-transcribed mRNA. NucleoBond PC 10000 EF is used during the culture, amplification, and purification stages to ensure the resulting plasmid DNA is free of harmful endotoxins.
NucleoBond PC 10000 EF can be used in the following applications:
- Adeno-associated virus (AAV) production
- Gene therapy research
- Vaccine development
NucleoBond PC 10000 Rack overview
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Demo video: NucleoBond PC 10000 and NucleoBond PC 10000 Rack
Learn how the NucleoBond PC 10000 kits can help in large-scale purification of up to 10 mg of ultrapure, endotoxin-free plasmid DNA.
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