In-Fusion Cloning citations
In-Fusion Cloning has been successfully used for seamless, easy cloning of multiple fragments, synthetic DNA fragments, lentiviral vector constructs, and guide RNA constructs for CRISPR/Cas9 gene delivery systems—and has proved valuable in high-throughput workflows. This technology has also been used for studying human disease, embryogenesis/development, microbiology, plant biology, and in the research against COVID-19 and the SARS-CoV-2 virus.
COVID-19
In-Fusion Cloning has been used to investigate and develop tools against COVID-19 and the SARS-CoV-2 virus that causes it.
- An infectious cDNA clone of a growth attenuatedKorean isolate of MERS coronavirus KNIH002 inclade B. Read now »
- Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections. Read now »
- Cutting Edge: Mouse SARS-CoV-2 Epitope Reveals Infection and Vaccine-Elicited CD8 T Cell Responses. Read now »
- Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza. Read now »
- Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV. Read now »
- Neutralization of SARS-CoV-2 by destruction of the prefusion spike. Read now »
- Neutralizing antibodies targetingthe SARS-CoV-2 receptor binding domain isolated fromanaïve human antibody library. Read now »
- Newcastle disease virus (NDV) expressing the spike protein of SARS-CoV-2 as a live virus vaccine candidate. Read now »
- Stability of RNA sequences derived from the coronavirus genome in human cells. Read now »
- Ubiquitin ligase RIPLET mediates polyubiquitination of RIG-I and LGP2 and regulates the innate immune responses to SARS-CoV-2 infection. Read now »
CRISPR/Cas9 vector cloning
In-Fusion Cloning has been used for seamless cloning of sgRNA constructs for CRISPR/Cas9 vector systems.
- Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza. Read now »
- Microhomology-assisted scarless genome editing in human iPSCs. Read now »
- A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants. Read now »
- Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system. Read now »
- MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Read now »
- The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation. Read now »
- Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Read now »
- Efficient genome editing in the oomycete Phytophthora sojae using CRISPR/Cas9. Read now »
- Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated gene editing. Read now »
High-throughput cloning in antibody engineering workflows
In-Fusion Cloning has been used for streamlined, high-throughput cloning in antibody engineering workflows.
- Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. Read now »
- Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains. Read now »
- Efficient generation of monoclonal antibodies from single rhesus macaque antibody secreting cells. Read now »
- Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis. Read now »
Lentiviral plasmid cloning
In-Fusion Cloning has been used for cloning inserts directly into large, complex lentiviral vectors.
- Gene expression profiling reveals U1 snRNA regulates cancer gene expression. Read now »
- Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Read now »
- Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Read now »
- Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors. Read now »
Human disease
In-Fusion Cloning has been used to investigate the mechanisms of various human diseases.
- Oxidative stress inhibits distant metastasis by human melanoma cells. Read now »
- Rb suppresses human cone-precursor-derived retinoblastoma tumours. Read now »
- Visualization and targeting of LGR5+ human colon cancer stem cells. Read now »
- DNA methylation analysis of the EGR3 gene in patients of schizophrenia. Read now »
Embryogenesis/development
In-Fusion Cloning has been used to study embryogenesis and development in different species.
- Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development. Read now »
- DUX-family transcription factors regulate zygotic genome activation in placental mammals. Read now »
Microbiology
In-Fusion Cloning has been used to study gene regulation, protein interactions, and metabolic pathways in various microorganisms, as well as viral infection of mammalian cells.
- Revisiting the regulation of the primary scaffoldin gene in Clostridium thermocellum. Read now »
- Yeast dynamin interaction with ESCRT proteins at the endosome. Read now »
- A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea. Read now »
- Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome. Read now »
Plant biology
In-Fusion Cloning has been used to study gene regulation, growth mechanisms, viral infections, and stress response in plants.
- Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition. Read now »
- Co-option of a photoperiodic growth-phase transition system during land plant evolution. Read now »
- Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase. Read now »
- Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones. Read now »
- DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response. Read now »
- A highly efficient ligation-independent cloning system for CRISPR/Cas9-based genome editing in plants. Read now »
High-throughput cloning
In-Fusion Cloning has been adapted to high-throughput cloning platforms to generate many constructs in parallel.
- A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Read now »
- Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. Read now »
- High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses. Read now »
Easily design primers for In-Fusion Cloning
Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.
Design your primersTakara Bio USA, Inc.
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